Mark I Model 68A Rodent/Cell line Gamma Irradiator

描述(申请人提供)：本意见书的目的是申请资金购买一台Mark I型号68A型啮齿动物铯(137Cs)辐照器，供几位PHS资助的科学家使用，这些科学家需要这项专门设备来照射小鼠或细胞系，作为其研究的关键组成部分。这些科学家对转基因小鼠的照射是进行免疫功能正常小鼠骨髓移植的先决条件，本研究的目的是：1.研究特定骨髓细胞群在动脉粥样硬化或心肌梗死病变发展过程中的作用2.研究各种黏附分子在动脉粥样硬化和心肌梗死炎症细胞正常运输和迁移中的生物力学贡献，其中双基因或三基因敲除通常对小鼠是致命的。3.引入胸腺前体细胞，将其导入受照宿主体内，使发育中的胸腺细胞c-myb基因缺失。这样做是为了测试c-myb在胸腺正负选择过程中的作用，比培育靶向c-myb基因座以及必要的Cre转基因到各种小鼠背景中更有效。4.在流感和呼吸道合胞病毒感染的小鼠模型中，研究克隆群体T淋巴细胞在诱导肺损伤中的作用5.以一种可重复的方式诱导肠粘膜损伤，以探讨各种细胞因子和生长因子在粘膜炎症和修复中的作用。在下列类型的研究中，细胞系的照射是必要的：1.生产维持培养的饲养细胞，如胚胎干细胞；2.对细胞系诱导明确的DNA损伤研究各种治疗的增敏效应以诱导辐射敏感性用于开发新的癌症治疗策略该辐照仪将取代现有的于1984年购买的Nordion Gamma Cell 40单元，其修复部件不再可用于商业。

DESCRIPTION (provided by applicant): The purpose of this submission is to request funding to purchase a Mark I Model 68A rodent cesium (137Cs) irradiator for use by several PHS funded scientists that require this item of specialized equipment to irradiate mice or cell lines as a critical component of their research. The irradiation of transgenic mice by these scientists is a pre-requisite to bone marrow transplantation in immune competent mice when the intent of the research is to: 1. Study the contribution of specific bone marrow cells populations to the progression of lesion development in atherosclerosis or myocardial infarction 2. Study the biomechanical contribution of various adhesion molecules in the normal trafficking and migration of inflammatory cells in atherosclerosis and myocardial infarction where the double or triple gene knockouts would normally be lethal to the mice. 3. To introduce thymic precursors that engineered to delete the gene encoding c-myb in developing thymocytes after introduction into irradiated hosts. This is done in order to test the role of c-myb during positive and negative selection in the thymus more efficiently than breeding the targeted c-myb locus, along with the necessary Cre transgenes onto a variety of mouse backgrounds 4. Investigate the contribution of clonal populations T-lymphocytes to the induction of pulmonary damage in mouse models of influenza and respiratory syncytial virus infection 5. Induce damage to the intestinal mucosa in a manner that is reproducible to investigate the role of various cytokines and growth factors in mucosal inflammation and repair. The irradiation of cell lines is necessary in the following types of studies: 1. Produce feeder cells for maintaining cultures a fastidious cell lines such as embryonic stem cells 2. Induce defined DNA damage to cell lines to study the sensitizing effects of various treatments to induce radiation sensitivity for the development of novel cancer therapeutic strategies This irradiator will replace an existing unit Nordion Gamma Cell 40 purchased in 1984 and for which repair parts are no longer commercially available.