Purchase of a Bruker EMX EPR spectrometer

描述(申请人提供)：申请购买电子顺磁共振(EPR)光谱仪的资金。新的光谱仪将取代现有的IBM/Bruker ER 200D系列光谱仪，该系列光谱仪于1982年购买，最近已出现故障。虽然我们已经能够通过借用微波桥暂时恢复机器，但越来越多的组件不再起作用，例如，我们不能再使用快速扫描。布鲁克不再支持如此陈旧的光谱仪，这一事实加剧了问题。这一仪器已经连续使用了20多年，出版了40多份出版物，显然已经到了使用寿命的尽头。因此，我们需要一台新机器。所要求的光谱仪是布鲁克EMX系列光谱仪，是对现有光谱仪的替代。这台新机器的一个重要好处是它将把灵敏度提高约4-10倍。虽然最初是由库克实验室购买的，但现有的光谱仪现在已经成为真正的共享仪器，目前正在罗杰·库克博士、罗恩·瓦尔博士、罗伯特·弗莱特里克博士、乔纳森·魏斯曼博士、爱德华·佩特博士、大卫·阿加德博士、保罗·奥蒂斯·德·蒙特拉诺博士和霍华德·迈巴赫博士的实验室使用。所有这些调查人员都是这项提议的一部分，除了一人之外，所有人目前都得到了NIH的资助。该小组的应用包括肌球蛋白、基于微管的马达蛋白、人类雌激素受体和普里恩的结构研究。此外，我们还将调查表皮的性质和血红素蛋白产生的自由基，核心使用者将成立一个咨询委员会来监督该仪器的使用并制定政策。加州大学旧金山分校将提供操作该仪器的空间，并提供资金支付意外维修费用。

DESCRIPTION (provided by applicant): Funds are requested for an electron paramagnetic resonance (EPR) spectrometer. The new spectrometer will replace an existing IBM/Bruker ER 200D series spectrometer that was purchased in 1982 and has recently broken down. Although we have been able to temporarily resurrect the machine by borrowing a microwave bridge, an increasing number of components no longer function, e.g., we can no longer use the fast sweep. The problems are exacerbated by the fact that Bruker no longer supports spectrometers that are this old. This instrument has been in continual use for more than 20 years, generating more than 40 publications and is clearly at the end of its useful lifetime. Thus, we are requesting a new machine. The spectrometer requested, a Bruker EMX series, represents a replacement of the existing spectrometer. An important benefit of the new machine is that it will increase sensitivity by about a factor of 4-10. Although originally purchased by the Cooke laboratory the existing spectrometer has now become a truly shared instrument that is currently being used by the laboratories of Dr. Roger Cooke, Dr. Ron Vale, Dr. Robert Fletterick, Dr. Jonathan Weissman, Dr. Edward Pate, Dr. David Agard, Dr. Paul Otiz de Montellano, and Dr. Howard Maibach. All of these investigators are a part of this proposal, and all but one are currently funded by the NIH. Applications of the group include structural studies of: myosin, the microtubule-based motor proteins, human estrogen receptors, and prions. In addition we will also investigate the properties of epidermis and generation of free radicals by heme proteins, The core users will form an advisory committee to oversee use of the instrument and to set policy. UC San Francisco will provide space for operating the instrument, and funds to cover unexpected repairs.