Investigating the Functional Role of Hydroxymethylation in the Pathogenesis of B-cell Malignancies

学生将培养免疫表型和细胞分选的关键技能，特别是关注来自多个器官（如外周血、脾脏、扁桃体）的免疫细胞，生成用于5hmC分析的测序文库，基于化学的下拉技术（HMCP），使用各种Illumina仪器和计算生物学方法进行高通量测序，以定量全基因组5hmC水平，并将这些数据与其他调控和转录信息整合。

The student will develop key skills in immunophenotyping and cell sorting, particularly focusing on immune cells from number of organs (e.g. peripheral blood, spleen, tonsils), sequencing libraries generation for 5hmC analysis, chemical-based pull-down technologies (HMCP), high-throughput sequencing using a variety of Illumina instruments and computational biology approaches for the quantification of genome-wide 5hmC levels and the integration of these data with other regulatory and transcriptional information.