IMMUNOHISTOCHEMISTRY SHARED RESOURCE

免疫组织化学共享资源(ISR)对于CCSG来说是新的，但它的服务在另一个VUMC研究中心的支持下已经提供了近十年。许多癌症研究人员使用这种资源，它在小鼠组织和/或细胞切片中提供免疫组织化学染色。人们普遍认识到，要想利用近年来出现的大量抗体，就需要制定标准化的免疫方案。ISR集中了这项任务，防止了不必要的重复，并允许访问国家认证的人员和最先进的设备。简而言之，ISR通过使用即用即用方案维护相关标记物(抗血清)库存；筛选/优化免疫组织化学抗血清；标准化 质量保证；以及提供组织微阵列的免疫染色。它将通过提供石蜡包埋、冰冻包埋和切片、适合常规染色、免疫染色、原位杂交、激光捕获分歧和从档案块中提取RNA的切片来支持最先进的显微标本的包埋、切片和染色；并提供定量形态计量分析技术的指导。莉莲·B·南尼博士是免疫组织化学共享资源中心的主任。她为这家合资企业带来了超过20年的显微镜、组织学和细胞生物学方面的研究和教学经验。她得到了一名实验室经理和其他技术人员的支持，这些技术人员随时可以联系到VICC调查人员。

The Immunohistochemistry Shared Resource (ISR) is new to the CCSG, but its services have been available for nearly a decade under the auspices of another VUMC research center. Many cancer investigators use this Resource, which offers immunohistochemical staining in mouse tissue and/or cellular sections. It is well recognized that the ability to make use of the large number of antibodies that have become available in Irecent years requires development of standardized immunoprotocols. The ISR centralizes this task and prevents needless repetition, and gives access to nationally-certified personnel and state-of-the-art equipment. In brief, the ISR supports cancer-related research by maintaining stocks of relevant markers (antisera) with ready-to-go protocols; screening/optimizing antisera for immunohistochemistry; standardizing quality assurance; and providing immunostaining of tissue microarrays. It will support state-of-the-art embedding, sectioning, and staining for microscopic specimens by providing paraffin embedding; frozen embedding and sectioning; sections suitable for routine staining, immunostaining, in situ hybridization, laser capture dissention and for RNA extraction from archival blocks; and the provision of guidance in techniques of quantitative morphometric analysis. Dr. Lillian B. Nanney is the Director of the Immunohistochemistry Shared Resource. She brings to this venture over 20 years of research and teaching experience in microscopy, histology and cell biology. She is supported by a lab manager and other technicians who are readily available to VICC investigators.