Transgenic Mouse

转基因小鼠共享资源（TMSR）为癌症中心的研究人员提供各种服务，用于生产和维护转基因和基因敲除小鼠。这些服务包括通过卵母细胞注射生产转基因小鼠，通过胚胎干细胞注射生产基因靶向小鼠，冷冻胚胎，重新衍生胚胎以消除病原体，培养胚胎干细胞用于基因靶向实验，以及协助对客户的小鼠进行基因分型和饲养。此外，TMSR目前维持两种在种系中表达Cre或FIp的专门的转基因系，用于体内转基因/敲除的遗传操作。TMSR拥有一个设备齐全的胚胎操作实验室， 博韦尔动物设施和毗邻的四个房间的小鼠饲养室套房。 自1999年成立以来，该资源已从28个构建体中产生了93个转基因创始小鼠，成功地用33个构建体靶向ES细胞，并成功地通过种系传递这些ES细胞系以产生敲除小鼠品系。此外，达特茅斯其他实验室培养的5个ES细胞克隆已被用于生产嵌合小鼠，这些小鼠通过生殖系传递ES细胞基因组。该设施已经冷冻了6个菌株，并重新衍生了12个菌株。NCCC研究人员对这一资源的利用正在迅速扩大，预计明年将有20个构建体成功用于制造转基因小鼠，12个敲除小鼠品系。随着癌症中心新教师的招聘，预计TMSR的使用将进一步增加。

The Transgenic Mouse Shared Resource (TMSR) provides a variety of services to Cancer Center investigators for the production and maintenance of transgenic and knockout mice. The services include production of transgenic mice by oocyte injection, production of gene targeted mice by embryonic stem (ES) cell injection, freezing of embryos, rederivation of embryos to eliminate pathogens; culturing ES cells for gene targeting experiments; and assisting with genotyping and husbandry of clients' mice. In addition, TMSR currently maintains two specialized transgenic lines that express Cre or FIp in the germline for genetic manipulation of transgene/knockouts in vivo. TMSR has a fully equipped embryo manipulation laboratory in the Borwell animal facility and an adjoining four-room suite of mouse husbandry rooms. Since its inception in 1999, this Resource has produced 93 transgenic founder mice from 28 constructs, has successfully targeted ES cells with 33 constructs, and has successfully transmitted these ES cell lines through the germline to produce knockout mouse strains. In addition, five ES cell clones cultured by other laboratories at Dartmouth have been used to produce chimeric mice that transmitted the ES cell genome through the germline. The facility has frozen six strains and rederived 12 strains. Use of this Resource by NCCC researchers is expanding rapidly, and it is predicted that 20 constructs will be successfully used to make transgenic mice and 12 knockout mouse lines will be made in the next year. Further increases in the use of the TMSR are anticipated with recruitment of new faculty to the Cancer Center.