Illumina ISCAN Microarray Reader

 描述（由申请人提供） 该提案汇集了盖泽尔医学院的2个独立共享资源，他们提议 为2个不同的客户共享ISCAN系统的使用。主要的将是DartMouse，一个共享的 资源，字面上服务于主要由NIH资助的研究人员在扫描全国客户 小鼠样本基因组中的SNP，以识别来自不同小鼠背景的遗传贡献区域。该服务用于1）快速同源（将转基因转移到不同的遗传近交背景中），2）背景检查（验证小鼠或小鼠细胞系中的实际小鼠背景）和3）小鼠GWAS研究。DartMouse最初是为达特茅斯研究人员提供的本地服务，并迅速响应其他学术用户的需求，因此我们现在已经为来自100多家机构的170名用户提供服务，并在过去7年中分析了5400多个基因组，目前每年使用超过1000个基因组。我们将目前的成功归功于我们是研究人员，我们真正了解学术研究人员及其在小鼠遗传学方面的需求。我们擅长的具体方面是：准确性和质量控制，实惠的价格，快速的周转，专有的数据分析程序，生成易于理解的每个染色体的菌株贡献的映射信息。所要求的仪器的第二个用户是基因组学和分子生物学共享资源（GMBSR），它提供各种基因组学服务。对Illumina iSCAN仪器特异的那些包括1）人转录组阵列，2）人甲基化阵列，3）小鼠GWAS阵列和4）人GWAS阵列。通过微阵列研究的转录组分析仍然是几个研究项目的主要应用。研究人员通常拥有历史数据集，并希望在未来的研究中继续使用相同的平台。由于相对较低的价格和快速的周转时间，微阵列非常适合高通量研究，如人类甲基化研究。达特茅斯基因组学和分子生物学共享资源（GMBSR）由生物信息学核心支持，由Chris Amos指导，转录组，GWAS和甲基化阵列数据的易于分析是进行此类研究的研究人员的主要考虑因素。因此，即使随着下一代测序的发展（我们也在达特茅斯提供），我们的微阵列业务仍然保持稳定，我们预计随着Illumina iSCAN的加入和提供新服务的能力，包括甲基化和GWAS功能，我们的业务将继续增长。

 DESCRIPTION (provided by applicant) This proposal bring together 2 separate shared resources at Geisel School of Medicine who propose to share usage of the ISCAN system for 2 different clientele. The primary will be DartMouse, a shared resource that literally serves a national clientele of primarily NIH funded researchers in scanning SNPs in genomes of mouse samples to identify regions of genetic contribution from different mouse backgrounds. The service is used for 1) speed congenics (transferring a transgene into a different genetic inbred background, 2) background checks (validating the actual mouse backgrounds in mice or mouse cell lines) and 3) mouse GWAS studies. DartMouse started as a local service for Dartmouth researchers and quickly responded to demand from other academic users so that now we have served 170 users from over 100 institutions and have assayed over 5400 genomes in the last 7 years with a current usage of over 1000 genomes per year. We attribute our current success to the fact that we are researchers and we really understand academic researchers and their needs in mouse genetics. Specific aspects at which we excel are: accuracy and quality control, affordable price, rapid turnaround, proprietary data analysis program that generates easy to understand mapping information of strain contribution to each chromosome.The second user of the requested instrument is the Genomics and Molecular Biology Shared Resource (GMBSR), which offer a variety of genomics services. Those that are specific to the Illumina iSCAN instrument include, 1) human transcriptome arrays, 2) human methylation arrays, 3) mouse GWAS arrays and 4) human GWAS arrays. Transcriptome profiling via microarray studies remains a main stay application for several research projects. Often researchers have historical data sets and wish to continue using the same platform for their future studies. Due to the relatively low price and quick turnaround times, microarrays are ideally suited to high throughput studies such as human methylation studies. Dartmouth Genomics and Molecular Biology Shared Resource (GMBSR) is supported by the Bioinformatics core, directed by Chris Amos, and the ease of analysis of transcriptome, GWAS and methylation array data is a major consideration for researchers undertaking these kinds of studies. Thus, even with the development of Next Generation Sequencing, (which we also offer at Dartmouth), our microarray business has remained steady, and we anticipate with the addition of the Illumina iSCAN and the ability to offer novel services, including methylation and GWAS capabilities that our business will continue to grow.