Genes & Gestational DM: Case Control Association Study

描述（由申请人提供）：妊娠期糖尿病（GDM）是在怀孕期间首次诊断的糖尿病;它发生在所有怀孕的7%。许多患有GDM的女性在以后的生活中被诊断为2型糖尿病（T2 DM）。某些基因与T2 DM相关，但尚未进行研究以确定这些基因是否与GDM相关。这项病例对照关联研究，匹配妇女的年龄，种族和体重指数，将检查GDM妇女与非GDM妇女的T2 DM相关基因。这些基因包括PPARgamma、B3-AR、GRL和CAPN 10。从血液样本中提取DNA。将使用聚合酶链反应扩增4种基因，并通过片段大小分析确定每份样本中存在哪些基因变体。卡方分析将比较GDM女性与非GDM女性个体基因变异的频率。本研究将有助于了解GDM和T2 DM的遗传学。与糖尿病相关的基因的鉴定可能有助于干预，以防止发病或改善与糖尿病相关的并发症。

DESCRIPTION (provided by applicant): Gestational Diabetes (GDM) is diabetes that is first diagnosed during pregnancy; it occurs in 7% of all pregnancies. Many women with GDM are diagnosed with Type 2 Diabetes (T2DM) later in life. Certain genes are associated with T2DM but studies have not been done to determine whether these genes are associated with GDM. This case-control-association-study, matching women for age, ethnicity and body mass index will examine genes associated withT2DM in women with GDM as compared to those without. These genes include the PPAR gamma, B3-AR, GRL and CAPN10. From blood samples, DNA will be extracted. Polymerase Chain Reaction will be used to amplify the 4 genes and fragment size analysis will determine which of the gene variants are present in each sample. Chi square analysis will compare the frequency of the individual gene variants in women with GDM vs. those without. This study will contribute to an understanding of the genetics of GDM and T2DM. Identification of genes associated with diabetes may facilitate interventions to prevent the onset or ameliorate the complications associated with diabetes.