Protein Interactions Involved in Poxvirus Envelopment

描述（由申请人提供）：无。（改编自申请）：这项工作的主要目标是确定六种蛋白质所采用的分子机制，这六种蛋白质是牛痘包膜形式产生包膜病毒体所特有的。这六种蛋白质之间的相互作用是有效包裹牛痘病毒所必需的假设将得到验证。这些相互作用将通过鉴定这六种蛋白质上的特异性结构域来描述，这些结构域是表达所需的，并测试这些鉴定的结构域与六种蛋白质的相互作用。这些区域的鉴定以及随后它们与什么相互作用不仅可以更好地理解这六种蛋白质的功能以及它们如何协调病毒介导，而且还将为进一步研究病毒介导机制奠定基础。将使用体内反式互补测定法鉴定特定结构域，其中将反式提供待测的特定蛋白质或蛋白质结构域，并使用活荧光显微镜目视评估互补作用。

DESCRIPTION (provided by applicant): None. (Adapted from the Application): The major goal of this work is to determine the molecular mechanism employed by six proteins that are unique to the enveloped form of vaccinia to produce enveloped virions. The hypothesis that interactions among these six proteins are required for efficient envelopment of vaccinia virus will be tested. These interactions will be delineated by identifying specific domains on these six proteins that are required for the envelopment and to test these identified domains for interaction with the six proteins. Identification of these regions and subsequently what they interact with will not only allow for a better understanding of the function of these six proteins and how they coordinate viral envelopment but will also lay the foundation for further studies into the mechanism of viral envelopment. Specific domains will be identified using an in vivo trans-complementation assay in which the specific protein or domains of the protein being tested will be provided in-trans and complementation will be assessed visually using live fluorescent microscopy.