Dissecting actin mediated nuclear destruction during keratinocyte terminal differentiation

在生物体的整个生命周期中，需要对细胞核进行有控制的破坏。尽管这一过程至关重要，但人们对其相关机制知之甚少，因为除了表皮角质形成细胞外，这是一个难以可视化的过程，表皮角质形成细胞经历核降解作为其终端分化过程的一部分。利用最先进的实时高分辨率光学显微镜，磷酸蛋白质谱和下一代RNA测序方法的结合，博士生将研究肌动蛋白重塑蛋白在核破坏中的作用，以及用药物诱导核破坏以激活这种肌动蛋白重塑途径的可能性。

Controlled destruction of the nucleus is required throughout the lifespan of an organism. Critical though this process is, little is known about the associated mechanisms as it is a difficult process to visualise except in epidermal keratinocytes, which undergo nuclear degradation as part of their terminal differentiation process. Using a combination of state-of the-art live high-resolution light microscopy, phosphoprotein mass spectrometry and next generation RNA sequencing approaches, the PhD student will examine the role of the actin remodelling proteins in nuclear destruction and the possibility of inducing nuclear destruction with drugs to active this actin remodelling pathway.