Microscopy of deformation processes in lipid bilayers

描述（由申请人提供）：这里描述的工作使用最先进的显微镜测量（光学和扫描探针）来检查脂质双层结构域的形成。特别关注的焦点是脂质结构对变形的反应。这项工作的三个具体目标包括：(1)微管支持解聚时囊泡的灾难性降解（这一过程与灵长类动物阿尔茨海默病的研究类似）；(2)脂液体积混合囊泡融合后三维成像方法；(3)表面支持脂质双层中蛋白-蛋白距离限制对结构域形成和细胞骨架生长模式的影响。

DESCRIPTION (provided by applicant): The work described here employs state-of-the-art microscopy measurements (optical and scanning probe) to examine domain formation in lipid bilayers. A particular focus of interest is in the response of lipid structure to deformation. Three specific targets of the work include: (1) catastrophic degradation of vesicles upon depolymerization of microtubule support (a process which has analogies in studies of Alzheimer's disease in primates); (2) lipid and fluid volume mixing in vesicle fusion followed by three-dimensional imaging methods; and (3) the effects of protein-protein distance constraints in surface-supported lipid bilayers on domain formation and cytoskeletal growth patterns.