Nanofibre adsorbent design informed by mechanistic modelling of bioprocess performance

该项目的目标是对与这种膜状支撑结构相关的理论及其在通过吸附捕获生物靶点和通过脱附洗脱的应用建立核心基本理解。其目的是引导输入到第二代Fibro技术平台。有了这些基本知识，将使用已知的生产方法在Cytiva工厂生产样品，以探索和验证这种理解。该项目将在很大程度上依赖于多因素实验设计方法，以确定影响后续性能特征的关键参数，如结合能力，背压和污垢。该项目的一个目标是将其纳入已知的机械模型，支持该技术的使用，并提供对改进设计的基本理解和预测。虽然大部分初步验证工作将利用模型蛋白和mAb，但该项目中建立的知识也旨在与病毒载体等更大的生物靶标相关。

This project will aim to build a core fundamental understanding of the theory relevant to this membrane-like support structure and its application in biological target capture via adsorption and elution via desorption. The aim being to guide input into the second generation of the Fibro technology platform. With this fundamental knowledge in place, known production methods will be used to generate samples at Cytiva facilities to explore and verify this understanding. The project will rely heavily on multi factorial Design of Experiment approaches to identify the key parameters that impact subsequent performance characteristics like binding capacity, back pressure and fouling. A goal of the project will be to include this into known mechanistic models which supports the use of the technology and provides fundamental understanding and prediction of improved designs. While much of the initial verification work will utilise model proteins and mAbs, the knowledge built in this project is also intended to be relevant for larger biologic targets like viral vectors.