MASS SPECTROSCOPY IMAGING OF PLANT-FUNGI INTERACTIONS: UNDERSTANDING SYMBIOSIS FOR SUSTAINABLE CROP PRODUCTION

这个项目的目的是了解植物如何为AM真菌提供脂质，它们使用什么类型的脂质以及这个过程是如何调节的。为了做到这一点，我们将结合植物分子遗传学方法和高空间分辨率质谱成像（MSI），使用斯旺西大学最先进的MSI仪器。MSI以前没有被用于研究AM共生，但这项技术有能力测量含有丛枝的单个根细胞的脂质组成。这项工作将为AM共生中的养分交换提供基础知识，可以为实现更可持续的作物生产提供新方法。

The aim of this project is to understand how plants supply lipids to AM fungi, what type of lipids they use and how this process is regulated. To do this we will combine plant molecular genetic approaches with high spatial-resolution mass spectrometry imaging (MSI), using state-of-the-art MSI instrumentation at Swansea University. MSI has not been used previously to study AM symbiosis, but this technique has the power to measure the lipid composition of individual root cells harbouring arbuscules. This work will provide foundation knowledge of nutrient exchange in AM symbiosis that can inform new approaches to achieve more sustainable crop production.