Core--Proteomic and Genomic Analyses

基因组学和蛋白质组学核心为涉及RNA和蛋白质水平基因表达全球概况的研究项目提供支持。核心主任和工作人员在全球基因表达谱的开创性方法方面有着悠久的历史，并为NCI资助的多研究者项目提供核心支持。岩心预计每年运行约25个样品，用于3种技术中的每一种的分析。对于小鼠研究，将从多只小鼠中合并样品，并重复对单个小鼠进行分析，以获得最高产量的实验。特定年份的精确混合和实验将取决于正在进行的研究的性质。例如，在第一年，小鼠样本的数量将高于人类样本。然而，在整个供资期间，核心员额的工作量预计相对稳定。除了大量成功的研究记录外，核心的一个主要优势是数据处理。该核心拥有广泛的数据处理资源，旨在实现标准化，将结果数据建立数据库，以及 探索性统计分析连续性统计分析将由项目统计人员进行。研究者可通过网站检索数据、研究报告，并下载在核心实验室实施的、可能对项目研究者有用的各种报告和方案。这些服务将包括： 1.提供微阵列分析，询问基因组中编码的大多数基因。 2.使用先进的样品制备程序和双向聚丙烯酰胺凝胶电泳提供蛋白质组学分析，该分析可检测组织样品中3，000种最丰富的蛋白质。 3.使用基于抗体的微阵列提供蛋白质组学分析， 该项目特别感兴趣的蛋白质， 微阵列或由Core生产的定制抗体微阵列。 4.提供蛋白质组学和基因组学数据的数据处理、加工和探索性统计分析方面的专业知识

The Genomics and Proteomics Core provides support for the program project for studies involving global profiling of gene expression at the RNA and protein levels. The Core Director and staff have a long history of pioneering approaches for global gene expression profiling and providing core support for NCI funded multi-investigator projects. The core expects to run approximately 25 samples per year for analysis by each of the 3 techniques. For mice studies, samples will be pooled from multiple mice, with repeat individual mouse analysis for experiments with the highest yield. Precise mixes and experiments in a given year will depend on the nature of ongoing studies. For example, in the first year mouse samples will be generated in a higher number than human samples. However the workload for the core is expected to be relatively constant throughout the funding period. A major strength of the core, in addition to a substantial track record of successful studies, is data handling. The core has extensive resources for data processing that are aimed at standardization, databasing of resulting data as well as exploratory statistical analysis. Definitive statistical analysis will be undertaken by Program Project statisticians. A website is available for investigators to retrieve data, study reports as well as for downloading a variety of write-ups and protocols that are implemented in the core laboratory and that may be of utility to project investigators. The services will include the following: 1. To provide microarray analyses that interrogate the majority of genes encoded in the genome. 2. To provide proteomic analysis using cutting-edge sample preparation procedures and two- dimensional polyacrylamide gel electrophoresis that interrogate the 3,000 most abundant proteins in a tissue sample. 3. To provide proteomic analysis using antibody based microarrays that assay several hundred proteins of particular interest to the project using either commercially available ready-to-use microarrays or custom-made antibody microarrays produced by the Core. 4. To provide expertise in data handling, processing and exploratory statistical analyses of proteomic and genomic data