CORE--Microarray and Bioinformatics

该核心B的微阵列部分将为鼠疫耶尔森氏菌、土拉热弗朗西丝氏菌和大肠杆菌的基因组制造斑点DNA微阵列。杆菌此外，该设施将根据需要制作多达5,000个基因的定制人类微阵列。该设施还将提供荧光扫描仪和计算机对阵列进行初步分析的设备，并将数据存款生物信息学设施维持的数据库。微阵列设施将有机会获得Affyphin基因芯片和Affyphin基因芯片扫描仪已经存在于斯托尼布鲁克。将使用Affytek芯片分析哺乳动物细胞对本项目中代表的三种A类因子感染的反应。分析 的Affymble数据将基本上与点样DNA微阵列相同。该核心的微阵列和生物信息学设施将在微阵列实验上密切合作。 核心的生物信息学部分将维护一组计算机，供本项目使用。将维护用于分析各种基因组数据的软件套件，用于序列比较和微阵列分析。该机构将维护数据库，用于存储微阵列结果，并用于感兴趣的生物体的基因注释。该核心将能够提供建议，并协助设计斑点DNA和Affyssin微阵列实验;它将协助解释微阵列实验;它将提供培训，编程支持和指导各种软件的使用 包装件.

The Microarray portion of this Core B will manufacture spotted DNA microarrays for the genomes of Yersinia pestis, Francisella tularensis, and E. coli. In addition, the facility will make custom human microarrays of up to 5,000 genes as needed. The facility will also provide equipment for the initial analysis of the arrays with fluorescent scanners and computers and will deposit the data in a database maintained by the Bioinformatics facility. The Microarray facility will have access to Affymetrix gene chips and an Affymetrix gene chip scanner already present at Stony Brook. Affymetrix chips will be used to analyze the response of mammalian cells to infection with the three Category A agents that are represented in this Project. Analysis of Affymetrix data will be essentially the same as for spotted DNA microarrays. The microarray and bioinformatics facilities of this Core will work closely together on microarray experiments. The Bioinformatics portion of the Core will maintain a cluster of computers for the use of this Project. Software suites for analysis of various kinds of genomic data will be maintained for sequence comparisons, and microarray analysis. The Facility will maintain databases for storage of microarray results, and for gene annotation of the organisms of interest. The Core will be able to provide advice and assist with the design of both spotted DNA and Affymetrix microarray experiments; it will assist in the interpretation of microarray experiments; and it will provide training, programming support, and guidance in the use of various software packages.