CORE--TRANSGENIC AND KNOCKOUT MOUSE CORE

转基因老鼠的使用呈指数增长。转基因和基因缺陷小鼠在研究糖尿病、内分泌紊乱、肥胖、糖尿病微血管和大血管并发症的机制以及阐明个体基因在正常生理、发育和内分泌功能中的作用方面得到了广泛的应用，具有重要的应用价值。事实上，对老鼠的基因操作几乎应用于生物医学研究的每一个领域。它可以说是解剖生理和病理生理中基因功能最有效的工具。然而，由于在个别实验室内建立这些程序所需的高昂费用和复杂技术，必须将这些技术作为核心设施加以支持。这需要一种高效且经济的通用设备，使个体研究人员能够同时拥有表达感兴趣基因的转基因小鼠和携带为它们创造的基因中断或替代的小鼠。本核心提供的技术将这些强大的分析工具置于所有人的手中

The use of genetically engineered mice has grown exponentially. Transgenic and genetically deficient mice are now widely used and extremely valuable for the study of the mechanisms of diabetes, endocrine disorders, obesity, and microvascular and macrovascular complications of diabetes as well as elucidating the roles of individual genes in normal physiology, development, and endocrine function. In fact, the genetic manipulation of mice is used in virtually every field of biomedical investigation. It is arguably the most potent tool available to dissect gene function in physiology and pathophysiology. However, because of the prohibitive costs and the technical sophistication required to establish these procedures within individual laboratories, it is essential to support these technologies as a core facility. This requires an efficient and cost-effective common facility that enables individual investigators to have both transgenic mice expressing genes of interest and mice carrying gene disruptions or substitutions created for them. The techniques available from this Core place these potent tools for analysis in the hands of all of our investigators. When used in combination with the other modern techniques supplied by the Center's Mouse Phenotyping, Transcriptional Genomics, and Biochemistry and Molecular Assay Core Core facilities, it gives our investigators unlimited opportunities to examine these processes from several perspectives simultaneously. Our current facility has an established track record of enabling the creation of both transgenic mice and knockout mouse strains for multiple UCSD laboratories. The availability of this Core facility to the entire DERC membership will greatly facilitate the research of the entire diabetes/endocrinology community in Southern California. Without such a centralized resource, most laboratories would not be in a position to utilize these powerful models, severely limiting the research and granting opportunities for our diabetes community. The Transgenic and Knock-out Mouse Core of UCSD is widely respected in the community and regarded as an essential resource for modern research. The availability of its services at substantially discounted rates will substantially improves the quality, quantity and creativity of the research, while also enhancina cost-effectiveness and efficiency.