Glycomics with Staudinger activated fluorogenic dyes

描述(由申请人提供)：本研究的最终目标是提供一种在各种环境中跟踪O-连接糖基化的简便方法。这将通过使用高度适应性的Staudinger连接活性荧光染料直接标记代谢性标记的O-葡聚糖来完成。合成的模块化性质将允许探测器的光物理、化学和物理特性的独立调节。双猝灭机构应提供极低的本底，便于与各种检测系统配合使用。到目前为止，还没有专门跟踪O连接糖基化的能力。最初，该探针将与2D凝胶电泳结合使用，以提供O-糖基化的“指纹”，并与质谱学技术相结合，将提供O-连接糖蛋白的身份。这种方法应该会极大地促进理解O-连接糖基化的影响和调节这种翻译后修饰的机制。

DESCRIPTION (provided by applicant): The ultimate goal of this research is to provide a facile method for tracking O-linked glycosylation in a variety of settings. This will be accomplished via the direct labelling of metabolically labelled O-glycans utilizing a highly adaptable Staudinger-ligation acitivatable fluorogenic dye. The modular nature of synthesis will allow independent tuning of photophysical, chemical and physical characteristics of the probe. A double quench mechanism should provide exceedingly low background, facilitating use with a variety of detection systems. The ability to specifically track O-linked glycosylation has, of yet, been unavailable. Initially this probe will be used in conjunction with 2D gel electrophoresis to provide a "fingerprint" of O-glycosylation and in conjunction with mass spectrometry techniques will provide the identity of O-linked glycoproteins. Such a method should greatly facilitate efforts to understand the impact of O-linked glycosylation and the mechnasims which regulate this post-translational modification.