Tracking Intracellular Transport of Self-Reporting Nanomaterials

CDT主题一致性：先进的产品设计合成和天然大分子的运输过程尚未完全理解，但支配着药物是否在正确的地点、正确的时间和正确的剂量被输送。该项目将着眼于新的合成和天然大分子和探针，用于实时报告交付情况。我们将使用FRET和相关的光学接近探头，在荧光标记的大分子和其环境的选定“地标”之间，在分子水平上获得关于生物环境(组织和细胞)定位的信息。该项目将包括新的响应性和可生物降解的聚合物递送系统的合成，荧光探针的结合，细胞/组织培养中递送系统和货物运输的评估，以及可能的体内实验。最后，我们的目标是举例说明贩运的新材料和知识，以提高siRNA敲除在急性肾损伤(Aki)中的治疗效果。

CDT Theme alignment: Advanced Product Design Transport processes for synthetic and natural macromolecules are not fully understood, but govern whether a drug is delivered in the right place, at the right time, and the right dose. This project will look at new synthetic and natural macromolecules and probes for real-time reporting of delivery. We will use FRET and related optical proximity probes between fluorescently labelled macromolecules and selected 'landmarks' of their environment, to gain information on localization in biological settings (tissue and cell) at the molecular level. The project will encompass synthesis of new responsive and biodegradable polymer delivery systems, conjugation of fluorescent probes, evaluation of delivery system and cargo transport in cell / tissue culture, and potentially in vivo experiments. Finally, we aim to exemplify the new materials and knowledge of trafficking to enhance therapeutic efficacy of siRNA knockdown in acute kidney injury (AKI)