Influence of Inflammation on B burgdorferi Adaptation

描述（由申请方提供）：莱姆病螺旋体伯氏疏螺旋体维持在涉及蜱虫载体和哺乳动物的复杂地方病生命周期内。螺旋体适应这些不同的环境，部分是通过选择性基因表达。环境因素如温度、pH值和营养物质影响体外B。burgdorferi基因表达。对感染小鼠皮肤中脂蛋白基因表达的分析确定了B的两种显著不同的微环境。伯氏适应：幼稚状态和免疫状态。在幼稚状态下，B。伯氏菌表达116个脂蛋白基因，而在慢性感染期间仅表达其中的34个。B。Burgdorferi能够侵入哺乳动物宿主的几乎所有器官和组织，这些不同的微环境可能影响其脂蛋白基因表达。这种病原体引起小鼠心脏炎和关节炎，这与人类莱姆病的两种主要表现相似。尚不清楚炎症是否影响B。burgdorferi适应本课题研究了脂蛋白基因的组织差异表达及炎症对B脂蛋白基因表达的影响。将使用鼠模型研究伯氏螺旋体。为实现这些目标，将完成以下具体目标。 1.探讨B的组织差异表达。膀胱、心脏和关节感染过程中伯氏脂蛋白基因的变化; 2.观察炎症对B的影响。burgdorferi适应

DESCRIPTION (provided by the applicant): Borrelia burgdorferi, the Lyme disease spirochete, is maintained within a complex enzootic life cycle involving the tick vector and the mammal. The spirochete adapts to these diverse environments, in part, by selective gene expression. Environmental cues such as temperature, pH and nutrients influence in vitro B. burgdorferi gene expression. Analysis of the gene expression of lipoproteins in the infected murine skin has defined two remarkably different microenvironments for B. burgdorferi adaptation: naive status and immune status. In the naive state, B. burgdorferi expresses 116 of its lipoprotein genes while only 34 of them are expressed during chronic infection. B. burgdorferi is able to invade almost all organs and tissues of the mammalian host and these diverse microenvironments may influence its lipoprotein gene expression. This pathogen causes murine carditis and arthritis that parallel two major manifestations of human Lyme disease. It is unknown whether inflammation influences B. burgdorferi adaptation. In this project, the tissue differential expression of lipoprotein genes and the influence of inflammation on lipoprotein gene expression of B. burgdorferi will be investigated using the murine model. To achieve these goals, the following specific aims will be completed. 1. To investigate the tissue differential expression of B. burgdorferi lipoprotein genes in the bladder, heart and joint during murine infection; 2. To examine the influence of inflammation on B. burgdorferi adaptation.