Accelerating disease resistance breeding for diploid potato germplasm improvement

虫害和疾病降低作物产量，需要喷洒农用化学品。我们的目标是用遗传抗性取代农药，用于害虫和疾病控制。该项目涉及新的方法（https：//tinyurl.com/ycn4od6f），以了解和利用马铃薯多样性，利用新获得的自交亲和二倍体马铃薯品系进行抗病性。四倍体马铃薯品系携带有用的抗病虫害变异，但四倍体的遗传分析是困难的。利用适当的杂交，二倍体遗传学将用于定义、渗入二倍体，并克隆马铃薯卷叶病毒（PLRV）和线虫抗性基因，并将利用基因组编辑来加速将这些基因培育成有用的二倍体并改善马铃薯性状。CRISPR/Cas9编辑将用于二倍体马铃薯改良。

Pests and diseases reduce crop yields, requiring agrichemical sprays. We aim to replace agrichemicals with genetic resistance for pest and disease control. This project involves novel approaches (https://tinyurl.com/ycn4od6f) to understand and use potato diversity for disease resistance using newly available self-compatible diploid potato lines. Tetraploid potato lines carry useful variation for pest and disease resistance, but genetic analysis in tetraploids is difficult. Using appropriate crosses, diploid genetics will be used to define, introgress to diploids, and clone potato leaf roll virus (PLRV) and nematode resistance genes, and will use genome editing to accelerate breeding such genes into useful diploids and improve potato traits. CRISPR/Cas9 editing will be used for diploid potato improvement.