In situ Crosslinkable Hydrogels for Tissue Repair

描述(由申请人提供)： 怀俄明州研究创新公司将开发可原位交叉连接的水凝胶，称为合成细胞外基质(SECM)，用于软组织修复。用快速固化的生物相容性大分子单体混合物制备的自成型细胞交互SECM填充创伤性、外科或慢性缺陷的能力将具有重要的临床应用价值。这些材料将由化学修饰的透明质酸和硫酸软骨素制备，这两种物质是ECM的两个主要糖胺聚糖(GAG)成分。由交联型GAG衍生物制成的生物材料可产生多种生物灵感新材料。SECM的概念结合了ECM蛋白和GAG，我们建议证明在体内存在种子细胞和组织缺损处时可以发生交联。将对两种交联剂进行研究，并对所得到的材料与细胞生长的兼容性进行评估。原位交联材料将在动物模型中进行体内疗效评估。 建议的材料解决了外科护理和伤口修复方面未得到满足的需求。许多外科缺陷、创伤性伤口和深度慢性伤口如果不使用移植的天然或合成材料进行增强，就无法愈合。一种模拟细胞外基质、有利于细胞种植和生长的生物相容性水凝胶将有许多重要的临床应用。

DESCRIPTION (provided by applicant): Wyoming Research Innovations, Inc. will develop in situ cross-linkable hydrogels, called synthetic extracellular matrix (sECM), for soft tissue repair. The ability to fill a traumatic, surgical, or chronic defect with a self-molding cell-interactive sECM prepared with a fast-curing mixture of biocompatible macromonomers would have significant clinical utility. These materials will be prepared from chemically modified hyaluronic acid and chondroitin sulfate, which are two major glycosaminoglycan (GAG) components of the ECM. Biomaterials from cross-linked GAG derivatives produce versatile new bioinspired materials. The sECM concept incorporates ECM proteins as well as GAGs, and we propose to demonstrate that crosslinking can occur in the presence of seeded cells and in a tissue defect in vivo. Two crosslinking chemistries will be investigated and the resulting materials will be evaluated for compatibility with cell growth. In situ cross-linked materials will be evaluated for in vivo efficacy in an animal model. The proposed materials address an unmet need in surgical care and wound repair. Many surgical defects, traumatic wounds and deep chronic wounds fail to heal without augmentation using grafted natural or synthetic materials. A biocompatible hydrogel that mimics ECM and is conducive to cell seeding and growth would have many important clinical applications.