Novel Strategies to Improve Yeast as an Expression Platform

巴斯德毕赤酵母（Pichia pastoris，重新分类为Komagataella pastoris）是生物制药行业中已建立的蛋白质表达平台。它的价值在于强大和严格调控的启动子的可用性，其生长至非常高的密度，以及其每升培养物分泌克量重组蛋白的能力。尽管如此，仍有改进的潜力，特别是因为一些感兴趣的蛋白质难以在该系统中表达。该项目旨在开发旨在提高产品质量和效价的新策略。这是对Fujifilm Diosynth Biotechnologies（FDB）挑战的回应，以改善其毕赤酵母表达系统，这是其广泛能力的一部分，使合同工作能够从基因到最终重组蛋白原料药进行。它将包含在生物加工2.0卓越中心内，这是爱丁堡大学，曼彻斯特大学和约克大学，IBioIC和FDB之间的新伙伴关系。这是一个滚动的跨学科合作研究项目系列，以应对生物制品制造中的挑战。

Pichia pastoris (reclassified as Komagataella pastoris), is an established protein expression platform in the biopharmaceutical industry. It is valued for the availability of powerful and tightly regulated promoters, for its growth to very high densities and for its ability to secrete gram amounts of recombinant protein per litre of culture. Nevertheless, there is potential for improvement, especially as some proteins of interest are difficult to express in this system. This project seeks to develop novel strategies aimed at improving product quality and titre. It is a response to a challenge from Fujifilm Diosynth Biotechnologies (FDB) to improve their Pichia expression system, part of their broad range of capabilities enabling contract work to be performed from gene to final recombinant protein bulk drug substance. It will be encompassed within the Centre of Excellence in Bioprocessing 2.0, a new partnership between the Universities of Edinburgh, Manchester and York, the IBioIC and FDB. This is a rolling series of collaborative interdisciplinary research projects to address challenges in the manufacture of biologics.