Ultra-High Throughput, High Purity Stem Cell Isolation

描述(申请人提供)：有相当多的证据表明，造血干细胞和间充质干细胞移植在治疗包括淋巴瘤、神经母细胞瘤和多发性骨髓瘤在内的多种癌症中的应用。此外，HSC/MSC疗法可用于重建免疫系统，再生受损组织，并为许多自身免疫性疾病(如糖尿病、类风湿性关节炎和系统性红斑狼疮)提供修复受损细胞的基因。移植治疗成功应用的主要障碍之一是难以从采集的样本中快速获得纯干细胞样本。 我们研究的最终目标是开发一种用于干细胞采集的超高通量高纯度细胞分选机Gigasort。细胞分选机由几个平行的微流控通道组成，它们组合在一起产生的产量预计至少是当前一代细胞分选机的10倍，而不会影响样品纯度。目前的建议旨在开发一种可在所使用的并行通道数量上扩展的多通道分选机试验台。我们将建造试验台，并通过实验测量荧光珠和细胞的分选性能，包括从血液中收集的细胞。

DESCRIPTION (provided by applicant): There is considerable evidence for the application of transplantation of Hematopoietic and Mesenchymal Stem Cells in the treatment of a wide range of cancers including lymphoma, neuroblastoma, and multiple myeloma. In addition, HSC/MSC therapies can be applied to reconstitute the immune system, regenerate damaged tissues, and deliver genes to repair damaged cells for many auto-immune diseases such as diabetes, rheumatoid arthritis, and systemic lupus erythematosis. One of the major roadblocks in the successful application of transplant treatments is the difficulty in rapidly obtaining a pure sample of stem cells in the harvested sample. This ultimate objective of our research is the development of an ultra-high throughput high-purity cell sorter, Gigasort, for stem cell harvesting. The cell sorter consists of several parallel microfluidic channels that combine to produce throughput that is expected to be at least 10x that of current generation cell sorters in practice, without compromising sample purity. The current proposal is aimed at developing a multichannel sorter test bed that is extendable in the number of parallel channels used. We will build the test bed and experimentally measure the sorting performance for fluorescent beads and cells, including cells harvested from blood.