Developing new tools to investigate complex redox-active proteins

我打算研究呼吸酶复合物I的难以捉摸的机制，呼吸酶复合物I是ATP合成所必需的蛋白质，其功能障碍与衰老和许多神经退行性疾病（包括帕金森病）有关。为了获得对大型金属酶（如复合物I）的前沿见解，Roessler实验室开发的一种新技术将得到进一步应用和发展，该技术涉及电子顺磁共振（EPR）光谱和蛋白质膜电化学（PFE）的结合。

I intend to investigate the elusive mechanism of respiratory enzyme complex I, a protein that is essential for ATP synthesis and whose malfunction is associated with ageing and numerous neurodegenerative disorders including Parkinson's disease. In order to acquire cutting-edge insight into large metalloenzymes such as the complex I, a novel technique developed by Roessler lab will be applied and developed further, which involves the combination of electron paramagnetic resonance (EPR) spectroscopy and protein film electrochemistry (PFE).