INIA:Array Core

描述(由申请人提供)： 该核心将在三个地点设有设施，德克萨斯大学 奥斯汀(协调站点)，印第安纳大学医学院 印第安纳波利斯和位于丹佛的科罗拉多大学健康科学中心。 印第安纳州工厂的工作将部分得到UT的分包合同的支持 奥斯汀和科罗拉多州的活动将得到资金的支持 可从IMA以外的来源获得。这三个网站都已经做出了 对基因阵列技术的大量投资和MA提案受益 从这个基础设施。总体目标是使用微阵列技术来 定义预测或伴随过度的基因表达的变化 饮酒。这需要开发和验证符合以下条件的阵列 可以准确地测量大脑区域中大量RNA的水平 无论是老鼠还是大鼠。特别重要的是站点之间的重现性 对于阵列过程的所有步骤，从组织解剖到阵列数据 信息学。这将提供一种分子神经适应的测量方法， 将是全面的(对大量大脑基因的分析)和 在各个项目中保持一致。我们将同时使用商业(Affymetrix)和 定制的阵列可覆盖小鼠、大鼠和果蝇 NIA项目的基因组。

DESCRIPTION (provided by applicant): This Core will have facilities at three sites, the University of Texas at Austin (coordinating site), Indiana University School of Medicine in Indianapolis and the University of Colorado Health Sciences Center at Denver. Efforts at the Indiana site will be supported in part by a subcontract from UT Austin and activities at the Colorado site will be supported by funds available from sources other than the IMA. All three sites have made a considerable investment in gene array technology and the MA proposals benefit from this infrastructure. The overall goal is to use microarray technology to define changes in gene expression that either predict or accompany excessive alcohol consumption. This requires development and validation of arrays that can accurately measure levels of large numbers of RNAs from brain regions of both mice and rats. Of particular importance is reproducibility among sites for all steps of the array process, from tissue dissection to array data informatics. This will provide a measure of molecular neuroadaptation that will be comprehensive (analysis of a large number of brain genes) and consistent across projects. We will use both commercial (Affymetrix) and custom fabricated arrays to provide coverage of mouse, rat and drosophila genomes for NIA projects.