Multivalent display of antigens via site selective enzymatic modification

疫苗开发中的一个主要问题是结合疫苗开发中异质性的重要性。在这个项目中，学生将开发各种化学酶策略来合成具有明确标记化学计量比的同源位置特定标记的多聚体蛋白质。然后，这些将被改造成应用于结合模型抗原。然后将评估已定义的化学计量学中的特定部位结合对随机标记的影响。

A major question in vaccine development is the significance of heterogeneity in conjugate vaccine development. In this project, the student will develop a variety of chemoenzymatic strategies to synthesize homogenous site-specifically labelled multimeric proteins with defined labelling stoichiometry. These will then be adapted for application to conjugate model antigens. The effect of site-specific conjugation in defined stoichiometry to random labelling will then be evaluated.