RAT RESOURCE AND RESEARCH CENTER: CLONING TECHNOLOGY

描述(摘自申请者的摘要)：作为人类模型的大鼠 疾病对人类几乎每一个领域都做出了宝贵的贡献 医药。最近，在开发试剂方面取得了重大进展， 基因组信息，以及关键的种系修改技术 老鼠，包括制造转基因动物。有了这些工具，RAT 模型可以为理解分子做出前所未有的贡献 人类疾病的基础与新疗法的靶向开发 战略。不幸的是，实现这一目标有几个主要障碍 大鼠作为人类疾病模型的全部潜力：1)许多近亲交配和 研究人员无法获得转基因老鼠品系。而当 商业生产者维护常用老鼠品系的库存，较少 研究人员通常无法获得常用的菌株。2)当 个别研究人员开发并表征了许多突变的大鼠模型，他们 缺乏物质和财政资源来维持潜在的重要地位 菌株。3)目前还不可能在大鼠身上进行基因敲除。4)质量 对传染病往往缺乏控制。加在一起， 这些障碍证明了对资源中心的明确需求，该中心可以 为生物医学界提供优质大鼠品系并发展 发展大鼠作为模型所需的技术。这个项目的总体目标是 申请建立一个鼠类资源研究中心(RRRC)。这 中心将通过开发满足生物医学研究社区的需求 用于分发高质量、特征良好的近交系的基础设施， 杂交和基因工程大鼠(GER)给调查人员，要么作为活的 或作为超低温保存的种质，并通过进行创新研究 产生新的信息，这些信息将使RRRC和科学工作者受益 社区。为了实现这一目标，建立了一个多学科的 联盟：印第安纳大学低温生物学研究所(CRI)， 哈兰·斯普拉格·道利公司(HSD)，纪念教育研究所和 西北大学医学院的研究(CMIER)将开发一种 RRRC。这个独特的财团在每个领域都拥有广泛的专业知识 所需学科，包括进口、再衍生、育种、胚胎和 配子冷冻保存、基因分型、表型分析、传染病 实验室老鼠的监测和分布。RRRC将提供一个 不间断地向生物医学研究人员供应老鼠。研究项目 旨在改进大鼠核移植和建立大鼠基因敲除模型， 将对大鼠进行低温生物学和健康监测。结果将 使世界各地的调查人员受益。

DESCRIPTION (Adapted from the applicant's abstract): Rats as models for human disease have made valuable contributions to almost every field of human medicine. Recently, major advances have been made in developing reagents, genomic information, and critical germline modification techniques for the rat, including making transgenic animals. With these tools in place, rat models can make unprecedented contributions toward understanding the molecular basis of human disease and targeting development of new therapeutic strategies. Unfortunately, there are several major impediments to realization of the full potential of rats as models of human disease: 1) Many inbred and genetically engineered rat strains are not available to researchers. While commercial producers maintain inventories of commonly used rat strains, less commonly used strains are frequently unavailable to researchers. 2) While individual investigators develop and characterize many mutant rat models, they lack the physical and financial resources to maintain potentially important strains. 3) Gene knockouts are not currently possible in the rat. 4) Quality control with respect to infectious diseases is often lacking. Taken together, these impediments document the clear need for a resource center that can provide high quality rat strains to the biomedical community and develop techniques needed for advancement of rats as models. The overall goal of this application is to establish a Rat Resource & Research Center (RRRC). This center will meet the needs of the biomedical research community by developing the infrastructure for distributing high quality, well-characterized inbred, hybrid and genetically engineered rat (GER) to investigators, either as live rats or as cryopreserved germplasm, and by performing innovative research that generates new information which will benefit the RRRC and the scientific community. To accomplish this goal, an established multidisciplinary consortium: the Cryobiology Research Institute at Indiana University (CRI), Harlan Sprague Dawley Inc. (HSD), Memorial Institute for Education and Research (CMIER) at Northwestern University Medical School, will develop a RRRC. This unique consortium has extensive expertise in each of the disciplines needed, including importation, rederivation, breeding, embryo and gamete cryopreservation, genotyping, phenotyping, infectious disease monitoring, and distribution of laboratory rats. The RRRC will provide an uninterrupted supply of rats to biomedical investigators. Research projects aimed at improving rat nuclear transfer and developing rat knockout models, cryobiology, and health monitoring of rats will be performed. Results will benefit investigators worldwide.