CORE B--BLOOD CELL SEPARATION AND MHC TYPING

Core B是血细胞分离和MHC分型的Core。该设施由两部分组成：每一部分都有专门的通风柜、孵化器、液氮和零下80摄氏度的冰柜。第一部分完成从全血到PBMC、血浆和血清样品的所有样品处理以及这些样品的冷冻。根据项目的个别要求，Core B的第一部分还分离B、T、NK、NKT， Core B的第一部分进行MHC等位基因分型，包括补体分型和B细胞的EBV转化，生成、储存和维持B淋巴母细胞样细胞系。基本分型方案包括DNA PCR分型和HLA-A、B、C、DRB（根据需要包括高分辨率）、DQA、DQB、DPA、DPB寡核苷酸探针杂交，以及BF、C2、C4A、C4B蛋白分型。Core B的第2部分还根据需要进行ABO血型分型。

Core B is a blood cell separation and MHC typing Core. The facility comprises two parts: each has devoted hoods, incubators and liquid nitrogen and -80 degrees C freezers. The first part performs all sample processing from whole blood to PBMC, plasma and serum samples and the freezing of these samples. When required for individual requirements of the Projects, the first part of Core B also separates B, T, NK, NKT, of Core B carries out MHC allele typing, including complement typing, and EBV transformation of B cells to generate, bank and maintain B lymphoblastoid cell lines. The basic typing regimen includes DNA typing by PCR and hybridization with oligonucleotide probes of HLA-A, B, C, DRB (including high resolution as needed), DQA, DQB, DPA and DPB and protein typing for BF, C2, C4A and C4B. Part 2 of Core B also performs ABO blood group typing as needed.