Core--Exercise facility

描述(由申请人提供)： 这个核心的目标是提供补充的组织病理学和科学支持 通过免疫组化或原位定位蛋白质和/或mRNAs的研究项目 组织和培养细胞的杂交。詹姆斯·A·理查森博士将监督核心。这个 CORE在以下方面拥有成熟的专业知识和能力：1)组织的加工 常规石蜡包埋；2)取骨骼肌和心脏，闪速冷冻 方法：1)冰冻切片；3)标准组织学染色和特殊染色(如有需要)；4)ATPase纤维和其他酶组织化学；5)放射性同位素原位杂交；6)石蜡包埋小鼠胚胎文库的维护，用于原位杂交分析发育基因的表达；7)明场、暗场、相对照、荧光显微镜和图像分析。

DESCRIPTION (provided by applicant): The objective of this core is to provide complementary histopathologic and scientific support for the research projects by localizing proteins and/or mRNAs by immunohistochemistry or in situ hybridization in tissues and cultured cells. Dr. James A. Richardson will supervise the core. The core has an established history of expertise and competence in: 1) processing of tissues for routine paraffin embedding; 2) harvesting and flash-freezing of skeletal muscle and heart for cryotomy; 3) the standard histologic stains and special stains (Masson?s Trichrome, PAS, Feulgen) when required; 4) ATPase fibertyping and other enzyme histochemistry; 5) radioisotopic in situ hybridization; 6) maintenance of a library of murine embryos embedded in paraffin for analysis of developmental gene expression by in situ hybridization; and 7) bright-field, dark-field, phasecontrast, and epifluorescence microscopy and image analysis.