Exploring the Scope and Mechanism of Immune-Suppression by a Novel P-Rex1 Interacting Protein

首先，学生将测试PIP缺陷和PIP/Prex1缺陷中性粒细胞通过趋化性和趋化运动迁移的能力。该学生将使用从野生型、PIP缺陷和PIP/Prex1缺陷小鼠中分离的中性粒细胞来评估受体运输、主要信号通路和效应反应，采用各种技术，如成像、western blotting和流式细胞术。S/他将使用亲和试剂和质谱鉴定中性粒细胞中PIP的分子靶点。S/he还将使用灌洗、组织学和活体显微镜等方法评估中性粒细胞向发炎和感染组织的募集以及杀死病原体的能力。因此，他/她将成为信号、贩运、白细胞生物学和先天免疫方面的专家。此外，他/她的发现可能对未来炎症性疾病的治疗策略有重大影响，这些疾病是由过度招募和活化的中性粒细胞加剧的

Initially, the student will test the ability of PIP-deficient and PIP/Prex1 deficient neutrophils to migrate by chemotaxis and chemokinesis. The student will use isolated neutrophils from wild-type, PIP-deficient and PIP/Prex1 deficient mice to evaluate receptor trafficking and the principal signalling pathways and effector responses, employing various techniques such as imaging, western blotting and flow cytometry. S/he will use affinity reagents and mass spectrometry to identify molecular targets of PIP in neutrophils. S/he will also assess neutrophil recruitment to inflamed and infected tissues and the capacity to kill pathogens, using methods such as lavages, histology and intravital microscopy. Thus, s/he will become an expert in signalling, trafficking, leukocyte biology and innate immunity. Moreover, his/her findings may have significant impact on future treatment strategies in inflammatory diseases that are exacerbated by excessively recruited and activated neutrophils