POROUS POLYMER RODS AS CHROMATOGRAPHIC OR REACTIVE MEDIA

描述(申请人提供)：这项延续方案旨在设计、制备和测试毛细管和片状的全新整体柱，这将为生物活性分子的研究、分离、分离、检测和鉴定提供无与伦比的潜力，并能够充分开发生命科学研究的丰富潜力。这些新的色谱柱将通过简单的自由基聚合过程来制备。优选实施方案将包括制备通用型大孔整体式聚合物，该聚合物具有针对所需应用优化的多孔特性。然后，利用紫外线引发的光接枝，孔表面将被提供各种高密度功能，包括疏水、亲水、可电离和反应部分。这种高度通用的方法也将被用来创造具有前所未有的连续和分层表面化学的整体。聚合和接枝这两个制备步骤都是独立、简单、无浪费、劳动强度较低的，并使各种定制的整体材料得以快速发展。整体毛细管柱将专门设计用于微反相色谱、毛细管和薄层电色谱的高通量和/或多维分离。利用微反相色谱柱，我们将利用精心设计的选择性色谱柱，探索分离难分离的蛋白质混合物。由于接枝化学的进步，全新的CEC分离模式将被开发出来，并用于蛋白质和多肽的快速和有效分离。利用大孔聚合物薄层层析和相关方法的新方法将集中在生物大分子的更大规模和二维分离上，并将包括加压和电驱动流动。该提案还着眼于开发可用于处理生物样本的增强型整体式工具。利用顺序光嫁接的优点，将在电喷雾发射器的尖端准备整体式装置，集样品采集、蛋白质的酶消化和多肽的分离为一体。该设备与质谱仪的直接耦合将简化和加速蛋白质组学研究。总体而言，我们的目标是在新领域扩大具有增强性能和意想不到的能力的整体式材料的应用，并在多个领域展示其巨大的潜力。

DESCRIPTION (provided by applicant): This continuation proposal aims at the design, preparation, and testing of completely new monolithic columns in capillary and sheet formats that will provide unmatched potential for the study, isolation, separation, detection, and identification of biologically active molecules and enable to fully exploit the rich potential of studies in life sciences. These new columns will be prepared via a simple free radical polymerization process. The preferred implementation will include preparation of a generic macroporous monolithic polymer with porous properties optimized for the desired application. The pore surface will then be provided with a wide variety of high-density functionalities including hydrophobic, hydrophilic, ionizable, and reactive moieties using UV initiated photografting. This highly versatile approach will also be used to create monoliths with unprecedented sequential and layered surface chemistries. Both preparation steps - polymerization and grafting - are independent, simple, waste-free, as well as less labor intensive, and enable the rapid development of a wide variety of tailor-made monolithic materials. Monolithic capillary columns will be specifically designed for the high throughput and/or multidimensional separations in microHPLC, as well as in capillary and thin layer electrochromatography. Using microHPLC, the separation of difficult protein mixtures will be explored using columns with carefully designed selectivities. Entirely new CEC separation modes enabled by the progress in grafted chemistries will be developed and used for the rapid and efficient separations of proteins and peptides. New approaches to thin layer chromatography and related methods making use of macroporous polymer sheets will be focused on the larger scale and 2-D separation of biomacromolecules and will include both pressure and electrodriven flow. This proposal also targets the development of an enhanced array of monolithic tools available for handling biological samples. Using the benefits of sequential photografting, monolithic devices will be prepared in the tip of electrospray emitters that will integrate sample collection, enzymatic digestion of proteins, and separation of peptides. Direct coupling of this device to mass spectrometer will simplify and accelerate proteomic research. Overall, our targets are to extend the applications of monolithic materials with both enhanced performance and unexpected capabilities in new territories and to demonstrate their vast potential in numerous areas.