Neurochemical and Behavioral Correlates of ETOH Effects

描述(由申请人提供)： 这是一项关于继续提供培训补助金的建议，以支持六个 博士后和三名博士后研究员将广泛和深入地 受训进行酒精中毒的研究。培训区域横跨 包括分子生物学和遗传学在内的最先进的方法， 电生理学、细胞成像、神经化学和行为学。研究 模特包括动物和人类。培训计划将促进和 支持合作研究。分子生物学培训将包括 配体门控离子通道在细胞表达中作用的研究 系统(Mihic，Harris)，开发新的转基因动物模型(Harris， Mihic)，以及乙醇反应基因的鉴定(Iyer，Bergeson， 哈里斯，米希克)。遗传学培训将包括对人类和动物进行基因分型。 受试者(弗罗姆、哈里斯、伯格森、莱尔)。电生理和 显微成像训练将包括全细胞实验和 单细胞和脑切片的细胞内方法(Morrisett，Mihic， 哈里斯)。神经化学方面的培训将集中在细胞内和 侧重于磷酸化的细胞外信号机制 谷氨酸、多巴胺和其他物质的释放和运输。 神经递质(Gonzales，Harris)和突触蛋白的表达 (莱斯利，莫里塞特)酒精对行为影响的研究培训 (运动技能、强化、焦虑和退缩)将会完成 (，Duvuchelle，Harris，Gonzales，Bergeson)心理社会方面的培训 酒精研究(Fromme)以及基因和酒精的相互作用 在人类(Fromme、Bergeson、Harris、Lyer)中也将进行饮酒。这个 培训计划将继续强调专业发展 (提高口头和书面交流技能)(埃里克森)。 博士后研究员将在培训开始时选择一个项目。 博士前实习生将轮流在选定的教职员工的实验室中工作 会员在选择学位论文研究的导师之前。博士前 学生将被要求完成一系列核心课程要求 包括乙醇对中枢神经系统的作用，科学伦理， 实验设计，并进行统计分析。我们将继续专注于 少数族裔招募。培训计划有着出色的记录，并将 继续专注于生产合格的酒精研究人员。

DESCRIPTION (provided by applicant): This is a proposal for the continuation of a training grant to support six predoctoral and three postdoctoral fellows who will be broadly and intensively trained to conduct research on alcoholism. Training areas span the breadth of state-of-the-art approaches including molecular biology and genetics, electrophysiology, cellular imaging, neurochemistry, and behavior. Research models include both animal and human. The training program will promote and support collaborative research. Molecular biology training will encompass studies of the function of ligand-gated ion channels in cellular expression systems (Mihic, Harris), development of new transgenic animal models (Harris, Mihic), and identification of ethanol responsive genes (Iyer, Bergeson, Harris, Mihic). Genetics training will involve genotyping of human and animal subjects (Fromme, Harris, Bergeson, lyer). Electrophysiological and microscopic imaging training will comprise experiments with whole cell and intracellular methods in single cells and in brain slices (Morrisett, Mihic, Harris). Training in neurochemistry will focus on intracellular and extracellular signaling mechanisms with an emphasis on phosphorylation (Morrisett, Leslie), release and transport of glutamate, dopamine, and other neurotransmitters (Gonzales, Harris), and expression of synaptic proteins (Leslie, Morrisett). Research training in the behavioral effects of ethanol (motor skills, reinforcement, anxiety, and withdrawal) will be done (,Duvauchelle, Harris, Gonzales, Bergeson). Training in psychosocial aspects of alcohol research (Fromme) and the interaction of genotype and alcohol drinking in humans (Fromme, Bergeson, Harris, lyer) will also be done. The training program will continue to emphasize professional development (improvement of oral and written communication skills) (Erickson). Postdoctoral fellows will choose a project at the onset of their training. Predoctoral trainees will rotate through the laboratories of selected faculty members prior to choosing an advisor for dissertation research. Predoctoral students will be required to complete a series of core course requirements covering ethanol's actions on the central nervous system, scientific ethics, experimental design, and statistical analysis. We will continue to focus on minority recruitment. The training program has an excellent record and will continue to focus on producing well qualified alcohol researchers.