High Throughput Screening of HIV Entry Inhibitors (RMI)

描述（由申请方提供）：人类免疫缺陷病毒（HIV）通过病毒包膜蛋白gp41和gp120与人靶细胞受体之间的特异性相互作用进入靶细胞。该项目的长期目标是发现小的药物样分子，通过与病毒包膜或人类靶细胞受体蛋白结合来破坏HIV进入。具体目标是：具体目标1：开发HIV病毒介导的病毒进入的假分型试验，用于通过机器人系统高通量筛选（HTS）小药物样分子。具体目标二：扩展HIV进入试验，以区分与HIV包膜蛋白结合的小分子抑制剂和与人靶受体蛋白结合的小分子抑制剂。

DESCRIPTION (provided by applicant): Human immunodeficiency virus (HIV) enters target cells by specific interactions between the viral envelope proteins gp41 and gp120 and human target cell receptors. The long term goal of this project is to discover small, drug-like, molecules that disrupt HIV entry by binding to viral envelope or human target cell receptor proteins. The specific aims are: Specific Aim 1: Develop the pseudo typing assay of HIV envelope-mediated viral entry for application to high throughput screening (HTS) of small drug-like molecules by robotic systems. Specific Aim 2: Extend the HIV entry assay for discrimination between small molecule inhibitors that bind to HIV envelope proteins, from small molecule inhibitors that bind to human target receptor proteins.