ADH and ALDH2 Genes in Tanzanian Populations

描述（由申请人提供）：这是一个项目的修订申请，该项目将主要由坦桑尼亚达累斯萨拉姆Hubert Kairuki纪念大学医学院生物化学和分子生物学系的Sylvester L.B. Kajuna博士在坦桑尼亚完成。它是与NIH拨款r01aa09379 - 09,5/1/03 -4/30/08相关的现有合作的延伸，但也涉及P01GM57672-06（3/1/04-2/28/09）。整个项目涉及研究初级乙醇代谢基因的遗传变异以及这些变异与酒精中毒易感性的关系。该项目的先前研究表明，ADH1B基因的一种功能变体（ADH1B*369Cys，以前称为ADH2*3）是非洲特有的，发生在几个非洲人群中，包括与Kajuna博士合作研究的Chagga。该提案旨在为Kajuna博士提供手术用品，以便他在达累斯萨拉姆的实验室可以更直接地参与分子工作，并可以研究坦桑尼亚的其他人群，以了解这种已知的功能变体，并可以寻找添加！这些变异可能存在于坦桑尼亚高度多样化的人群中。此外，有了当地的设施和供应，将有可能直接测试ADH1B*369Cys等位基因是否与防止酗酒的保护作用有关。与大多数国家一样，酗酒在坦桑尼亚是一个重大的公共卫生问题。该研究将与耶鲁大学正在进行的研究（AA09379）进行协调，并在耶鲁大学进行实验室研究的培训和方案制定，这些研究将在休伯特·凯鲁基纪念大学的Kajuna博士实验室进行。香港大学的实验室运作的另一个好处是，它可以为学生和初级研究人员提供分子方法学方面的实验室培训。

DESCRIPTION (provided by applicant): This is a revised application for a project which will be done primarily in Tanzania by Dr. Sylvester L.B. Kajuna, of the Department of Biochemistry and Molecular Biology, The Hubert Kairuki Memorial University Faculty of Medicine, Dar es Salaam, Tanzania. It is an extension of an existing collaboration related to NIH grant R01AA09379-09, 5/1/03-4/30/08 but also relates to P01GM57672-06 (3/1/04-2/28/09). The overall project involves studying the genetic variation in the primary ethanol metabolizing genes and the relationships of those variants to susceptibility to alcoholism. Previous studies on this project have shown that one functional variant of the ADH1B gene (ADH1B*369Cys, previously named ADH2*3)is African-specific and occurs in several African populations, including the Chagga studied in collaboration with Dr. Kajuna. This proposal is designed to provide Dr. Kajuna with operation supplies so that his laboratory in Dar es Salaam can be more directly involved in the molecular work and can study additional populations in Tanzania for this known functional variant and can look for addition! variants that might exist in the highly diverse populations of Tanzania. In addition, with local facilities and supplies it will be possible to test directly whether the ADH1B*369Cys allele is associated with a protective effect against alcoholism. As in most countries, alcoholism is a significant public health problem in Tanzania. The research will be coordinated with the ongoing studies at Yale (under AA09379) with training and protocol development taking place at Yale for laboratory studies to be undertaken in Dr. Kajuna's laboratory at The Hubert Kairuki Memorial Univeristy. An additional benefit of a functioning laboratory at HKMU is that it will allow laboratory training for student and junior researchers in molecular methodology that is not otherwise present.