ADH and ALDH2 Genes in Tanzanian Populations

描述(由申请人提供)：这是一个项目的修订申请，该项目将主要由坦桑尼亚达累斯萨拉姆休伯特·凯鲁基纪念大学医学院生物化学和分子生物学系的西尔维斯特·L·B·卡朱纳博士完成。它是与NIH拨款R01AA09379-09，5/1/03-4/30/08相关的现有合作的扩展，但也与P01GM57672-06(3/1/04-2/28/09)相关。整个项目包括研究主要乙醇代谢基因的遗传变异，以及这些变异与酒精中毒易感性的关系。以前对该项目的研究表明，ADH1B基因的一个功能变体(ADH1B*369Cys，以前命名为ADH2*3)是非洲特有的，存在于几个非洲种群中，包括与Kajua博士合作研究的Chagga。这项提议旨在为卡朱纳博士提供手术用品，以便他在达累斯萨拉姆的实验室能够更直接地参与分子工作，并可以研究坦桑尼亚更多的人口的这种已知功能变异，并可以寻找额外的！在坦桑尼亚高度多样化的人口中可能存在的变种。此外，利用当地的设施和用品，将有可能直接测试ADH1B*369Cys等位基因是否与防止酒精中毒的保护作用有关。与大多数国家一样，酗酒是坦桑尼亚的一个重大公共卫生问题。这项研究将与耶鲁大学正在进行的研究(根据AA09379)进行协调，耶鲁大学正在进行培训和方案制定，以便在休伯特·凯鲁基纪念大学Kajua博士的实验室进行实验室研究。香港理工大学实验室运作的另一个好处是，它将为学生和初级研究人员提供分子方法学方面的实验室培训，而这些培训是在其他方面不存在的。

DESCRIPTION (provided by applicant): This is a revised application for a project which will be done primarily in Tanzania by Dr. Sylvester L.B. Kajuna, of the Department of Biochemistry and Molecular Biology, The Hubert Kairuki Memorial University Faculty of Medicine, Dar es Salaam, Tanzania. It is an extension of an existing collaboration related to NIH grant R01AA09379-09, 5/1/03-4/30/08 but also relates to P01GM57672-06 (3/1/04-2/28/09). The overall project involves studying the genetic variation in the primary ethanol metabolizing genes and the relationships of those variants to susceptibility to alcoholism. Previous studies on this project have shown that one functional variant of the ADH1B gene (ADH1B*369Cys, previously named ADH2*3)is African-specific and occurs in several African populations, including the Chagga studied in collaboration with Dr. Kajuna. This proposal is designed to provide Dr. Kajuna with operation supplies so that his laboratory in Dar es Salaam can be more directly involved in the molecular work and can study additional populations in Tanzania for this known functional variant and can look for addition! variants that might exist in the highly diverse populations of Tanzania. In addition, with local facilities and supplies it will be possible to test directly whether the ADH1B*369Cys allele is associated with a protective effect against alcoholism. As in most countries, alcoholism is a significant public health problem in Tanzania. The research will be coordinated with the ongoing studies at Yale (under AA09379) with training and protocol development taking place at Yale for laboratory studies to be undertaken in Dr. Kajuna's laboratory at The Hubert Kairuki Memorial Univeristy. An additional benefit of a functioning laboratory at HKMU is that it will allow laboratory training for student and junior researchers in molecular methodology that is not otherwise present.