The genetics of reproductive isolation in Leavenworthia

18.奖学金培训的目标和职业名称(最后、第一个、中间的首字母)科林，Vanessa，A机构导师学院，波特兰，或Keith Karoly博士主管/雇主机构/公司Reed学院Keith Karoly博士作为NIH博士前研究员和佐治亚大学的博士候选人，我的主要目标将是研究植物Levenworth ia(十字花科)物种形成的生态学和分子遗传学。开展这项拟议的研究将使我能够在团契培训期间实现其他补充目标。这些相辅相成的目标包括学习新的现场和实验室技术，并在使用这些技术方面获得更多经验。特别是，我计划接受更广泛的分子遗传技术培训，以检查基因结构和功能、染色体结构和功能，以及整个基因组进化。分子遗传学技术将帮助我完成对物种形成遗传学的更多理解，而不是使用纯粹的生态遗传学方法。在我从佐治亚大学获得博士学位后，我打算继续研究物种形成的遗传学，同时在学院或大学一级教授进化遗传学课程。最终，我的奖学金培训和研究生教育将在我的智力发展和职业发展中发挥关键作用。-‘1I’Jl_‘L’II.姓名和学位(S)罗德尼·毛利西奥，博士20.遗传学助理教授研究兴趣/领域复杂性状的遗传和进化；群体遗传学、生态遗传学；进化遗传学il=_1_‘11i|’_：i“：{，li{，lql‘，22。描述(不超过所提供的篇幅)了解物种如何形成是进化遗传学的基本问题之一。这对理解基因组进化是至关重要的。回答这个问题需要了解物种间生殖隔离的遗传基础。具体的问题是：(1)哪些进化过程导致了物种间的生殖障碍；(2)物种间繁殖的某些障碍出现的频率是多少？(3)与物种间生殖隔离相关的数量性状有多少个基因座？(4)哪些基因控制物种之间的生殖隔离及其功能？为了开始回答这些问题，我们将确定利文沃斯属植物(L.alabamica和L.crassa)两个物种之间生殖隔离的生态学和遗传学基础。相互移植实验将确定是否存在生态地理隔离。将进行传粉者观察，以了解不同物种的传粉者偏好是否不同，这将是一种行为学上的孤立。阿拉巴马利文沃斯和粗毛利文沃斯在自然界中进行杂交，因此将检查杂种的生存能力，以确定是否存在任何合子后繁殖障碍。将绘制数量性状基因座图，以确定有多少遗传基因座负责生殖隔离。这些QTL将与拟南芥的序列进行比较，作为失去功能的线索。最后，在这些物种或它们的杂交后代中观察到的任何近亲繁殖衰退的遗传学将被确定。PHS 416-1(12/98版)表第2页bb cc个人非RSA申请目录========================================Section End===========================================

18. GOALS FOR FELLOWSHIP TRAINING AND CAREER NAME (Last, first, middle initial) Koelling, Vanessa, A. INSTITUTION MENTOR College, Portland, OR Dr. Keith Karoly SUPERVISOR/EMPLOYER INSTITUTION/COMPANY Reed College Dr. Keith Karoly As an NIH pre-doctoral fellow and Ph.D. candidate at the University of Georgia, my primary goal will be to study the ecological and molecular genetics of speciation in the plant genus Leavenworthia (Brassicaceae). Undertaking this proposed research will allow me to meet other complementary goals during my fellowship training as well. These complementary goals include learning new field and laboratory techniques, and gaining more experience in the use of those techniques. In particular, I plan to be more extensively trained in molecular genetic techniques for the examination of gene structure and function, chromosome structure and function, and genome evolution as a whole. Molecular genetic techniques will aid me in completing more comprehensi,m studies of the genetics of speciation than could be done using a purely ecological genetics approach. After I receive my doctoral degree from the University of Georgia, I intend to continue studying the genetics of speciation while teaching evolutionary genetics courses at the college or university level. Ultimately, my fellowship training and graduate education will play a pivotal role in my intellectual development and career growth. -'1i'Jl _'l'ii 19. NAME AND DEGREE(S) Rodney Mauricio, Ph.D. 20. POSITION/RANKAssistant Professor of Genetics 21. RESEARCH INTERESTS/AREAS The genetics and evolution of complex traits; population genetics, ecological genetics; evolutionary genetics il=_1_'11i|'_:I" :{,li{,lql', 22. DESCRIPTION (Do not exceed space provided) Understanding how species form is one of the fundamental unanswered questions of evolutionary genetics. And it is critical in understanding genome evolution. Answering this question requires knowledge of the genetic basis of reproductive isolation between species. Specific questions are: (1) what evolutionary processes are responsible for reproductive barriers between species?; (2) what are the frequency of certain barriers to reproduction between species'?.; (3) how many loci contribute to the quantitative traits associated with reproductive isolation between species?; and (4) what genes control reproductive isolation between species and how do they function? To begin to answer these questions, the ecological and genetic basis of reproductive isolation between two species in the plant genus Leavenworthia (L. alabamica and L. crassa) will be determined. Reciprocal transplant experiments will determine whether or not ecogeographic isolation is present. Pollinator observations will be conducted to see if pollinator preferences differ between species, which would be a form of ethological isolation. Leavenworthia alabamica and L. crassa hybridize in nature, and thus hybrid viability will be examined in order to determine if any post-zygotic barriers to reproduction exist. Quantitative trait loci will be mapped to determine how many genetic loci are responsible for reproductive isolation. These QTL will be compared to the sequence of Arabidopsis thaliana as a clue to gone function. Finally, the genetics of any inbreeding depression observed in these species or their hybrids will be determined. PHS 416-1 (Rev. 12/98) Form Page 2 BB cc Individual NRSA Application Table of Contents ========================================Section End===========================================