The acclimation of Chlamydomonas to sulfur deprivation.

描述（由申请人提供）：本提案描述了一系列将用于研究硫信号转导级联的组成部分的遗传和生化方法，这些信号转导级联负责莱茵衣藻对硫剥夺的驯化反应。最初的工作将产生一系列插入突变体，这些突变体缺乏对硫剥夺的正常反应。遗传分析将用于确定突变表型是否与插入分离。这些突变体的遗传损伤将被确定，并将通过将突变体转化为野生型位点来尝试互补。这些信息将用于生成硫酸盐信号转导级联使用遗传方法的模型。利用途径模型，生化方法将研究单个途径组分之间可能的相互作用，以及发现新的相互作用物。野生型和突变型菌株将用于生化研究，重点是确定哪些蛋白质参与细胞对硫剥夺的适应物理相互作用。这将有助于建立更详细的信号通路。

DESCRIPTION (provided by applicant): This proposal describes a series of genetic and biochemical approaches that will be used to study the components of the sulfur-signal transduction cascade that is responsible for the acclimation responses of Chlamydomonas reinhardtii to sulfur deprivation. Initial work will generate a collection of insertional mutants that lack the normal responses to sulfur deprivation. Genetic analysis will be used to determine if the mutant phenotype segregates with the insertion. The genetic lesions in these mutants will be determined and complementation will be attempted by transforming the mutants with the wild-type locus. This information will be used to generate a model for the sulfate signal transduction cascade using a genetic approach. Using the pathway model, biochemical methods will investigate the possible interaction between components of an individual pathway, as well as to discover new interactors. The wild-type and mutant strains will be used for biochemical studies focused on determining which of the proteins involved in the acclimation of cells to sulfur deprivation physically interact. This will help build a more detailed picture of the signaling pathway.