Development of combined bio- and chemo-catalytic cascades for the preparation active pharmaceutical ingredients (APIs)

该项目将从代谢途径中获取天然酶，并在必要时对其进行进化以扩大其底物范围。我们将联合收割机这些生物催化剂与兼容的化学催化剂，以扩大优化路线的合成效用。该项目将提供现代生物催化剂方法和分析方面的培训（重组DNA技术、酶特性鉴定和工程）。我们的目标是与X射线晶体学专家合作，指导目标酶的工程/进化。此外，坎波皮亚诺实验室还与工业生物催化团队建立了广泛的联系网络，我们将有机会扩大与他们的合作

This project will source natural enzymes from metabolic pathways and where necessary, evolve them to expand their substrate scope. We will combine these biocatalysts with compatible chemo-catalysts to expand the synthetic utility of the optimised route. This project will provide training in modern biocatalyst methodology and analysis (recombinant DNA technology, enzyme characterisation and engineering). We will aim to collaborate with experts in x-ray crystallography to guide engineering/evolution of target enzymes. Moreover, the Campopiano labs have built an extensive network of links with industrial biocatalysis teams and there will be an opportunity to expand our collaborations with them