Mechanisms of renal tubular epithelial cell injury

整体描述(由申请人提供) 急性肾功能衰竭后的总死亡率保持在 大约50%。这种高死亡率和相关成本不太可能 将会减少，直到我们对细胞和 细胞损伤和修复的分子机制。这项计划项目拨款 应用程序代表了基础应用程序之间的多学科协作 科学家和临床科学家围绕一个中心主题来阐明 肾小管上皮细胞损伤的细胞和分子基础 包括从细胞凋亡研究中学到的概念。 参与项目的有六个部门的成员(医学、 病理学、微生物学和免疫学、老年病学、生物化学和生理学) 包括几位在体外和体内都有专长的细胞生物学家 急性肾功能衰竭模型(Shah，Portilla，Walker，Safirstein)， 具有分子生物学专业知识的调查人员(Crew，Portilla，Haun， 普莱斯)，临床科学家，肾脏病理学家(沃克)，以及 在蛋白质纯化方面有特殊专长的生物化学家(Kaushal， Basnakian)。其他优势包括：1.一些原始的观察结果 从细胞凋亡研究中学到的概念与肾脏的相关性 本申请的参与者报告了肾小管损伤。2. 项目总监、项目负责人以及其他合作调查人员都有 专业协会和出版合作研究的历史。这个 所有项目负责人的实验室和办公室都非常接近 以允许参与者之间的随时访问和交互。 3.参与方案项目的几名调查人员以及 该机构药理学系的其他调查人员， 生理学和肾脏科正在进行的项目是 与这项赠款申请的中心主题直接相关，进一步 加强环境，以实现应用程序的目标。该计划 已经获得了足够多的研究人员来解决这种疾病的发病机制 以多方面、协同的方式治疗急性肾功能衰竭，从而与 计划项目指导方针所述的总体目标。

OVERALL DESCRIPTION (Provided by the applicant) The overall mortality following acute renal failure has remained at approximately 50%. It is unlikely that this high mortality and associated cost will be reduced until we have a better understanding of the cellular and molecular mechanisms of cell injury and recovery. This program project grant application represents a multidisciplinary collaborative effort among basic scientists and clinician scientists around a central theme of elucidating the cellular and molecular basis of rental tubular epithelial cell injury, including concepts that have been learned from the study of apoptosis. Participating in the projects are members of six departments (Medicine, Pathology, Microbiology & Immunology, Geriatrics, Biochemistry and Physiology) and include several cell biologists with expertise with in vitro and in vivo models of acute renal failure (Shah, Portilla, Walker, Safirstein), investigators with expertise in molecular biology (Crew, Portilla, Haun, Price), clinician scientist who is a renal pathologist (Walker), and biochemists with particular expertise in protein purification (Kaushal, Basnakian). Additional strengths include: 1. Some of the original observations of the relevance of the concepts learned from the study of apoptosis to renal tubular injury were reported by participants in this application. 2. The program director, project leaders as well as other co-investigators have a history of professional association and publishing collaborative studies. The laboratories and offices of all project leaders are located in close proximity to each other allowing ready access and interaction between the participants. 3. Several of the investigators participating in the program project as well as other investigators at the institution in the Departments of Pharmacology, Physiology and the Division of Nephrology have ongoing projects that are directly related to the central theme of this grant application which further strengthens the environment to pursue the goals of the application. The program has attained a critical mass of investigators to address the pathogenesis of acute renal failure in a multifaceted, synergistic fashion thus matching the overall stated objectives of the program project guidelines.