The Genetic Epidemiology of Asthma in Costa Rica

描述 (申请人？S摘要)这项建议旨在为 首席研究员(PI)获取遗传学方面的知识和技能 流行病学，在高资质赞助商的直接监督下， 进一步增强他发展成为独立调查员的潜力。 PI受过临床肺部和危重护理医学培训，拥有 最近获得了M.P.H.学位，除了完成呼吸系统 流行病学研究员资格和博士学位的初步要求 遗传流行病学。这项提案的头两年将包括 所需的课程作业、研讨会和基因分析和实践经验 分子生物学技术使PI能够进行密集的 建议后三年的研究经验。整体而言 这项建议的科学目标是研究基因对 哮喘及其相关表型在大样本和同质性研究中的发展 西班牙裔受试者的子集。我们假设染色体的一个子集 以前与哮喘相关表型相关的区域将有助于 哮喘在基因隔离的哥斯达黎加人群中的表达。至 检验这一假设，我们将对两条染色体进行定向筛选 区域(5q31-q33和12q15-q24.1) 在拉丁美洲血统的受试者中患有哮喘，这一提议可能会极大地 有助于我们了解哮喘的发病机制，特别是 在西班牙裔美国人中。此外，它还将创造一个表型和 基因类型信息，将有助于进一步的假设检验 独一无二的患者队列。

DESCRIPTION (Applicant?s abstract) This proposal seeks to provide an opportunity for the Principal Investigator (PI) to gain knowledge and skills in genetic epidemiology, under the direct supervision of a highly qualified sponsor, to further enhance his potential to develop into an independent investigator. The PI, who is trained in clinical Pulmonary and Critical Care Medicine, has recently obtained a M.P.H. degree, in addition to completing a respiratory epidemiology fellowship and the initial requirements for a Dr. P.H. degree in Genetic Epidemiology. The first two years of this proposal will incorporate needed course work, seminars, and hands-on experience in genetic analysis and molecular biology techniques to enable the PI to undertake an intensive research experience in the latter three years of the proposal. The overall scientific goal of this proposal is to study the genetic influences on the development of asthma and asthma-related phenotypes in a large and homogeneous subset of Hispanic subjects. We hypothesize that a subset of chromosomal regions previously linked to asthma-related phenotypes will contribute to the expression of asthma in a genetically isolated Costa Rican population. To test this hypothesis, we will conduct a directed screen on two chromosomal regions (5q31 - q33 and 12q15- q24.1) By identifying genetic determinants of asthma among subjects of Latin American ancestry, this proposal could greatly contribute to our understanding of the pathogenesis of asthma, particularly among Hispanics. In addition, it will create a bank of phenotypic and genotypic information that will facilitate further hypothesis testing in a unique patient cohort.