Dietary strategies to target adipose tissue inflamm

人口老龄化的人口结构变化是大多数国家面临的最大健康挑战之一。到2041年，英国近30%的人口将达到65岁。因此，找到改善老龄化和“健康寿命”的方法将是未来几十年的全球优先事项。我们最近的研究表明，老年人的脂肪组织是“促炎”的，即使人们保持苗条和体力活动。脂肪组织炎症影响健康的多个方面--包括慢性病的风险和急性感染的易感性。它还影响肌肉合成的能力，从而使人们容易患上骨质疏松症，从而导致虚弱和功能独立性降低。这项研究的目的是探索我们是否可以利用饮食来针对老年人的脂肪组织炎症和肌肉合成能力。这一博士学位将结合体内和体外的方法与高度复杂的分析方法。实验室技术将包括：细胞培养、先进的12色免疫表型和用流式细胞术(免疫学)测量细胞功能、多重分析、分子技术(例如RT-PCR、免疫印迹)和稳定同位素(示踪剂)。这些研究将在人类身上进行，将包括干预研究(随机对照试验)；采用复杂的组织特异性(例如脂肪和肌肉活组织检查)和全身(例如蛋白质周转)测量。还将有机会利用现有的RNAseq数据集开发来自老年人的脂肪和肌肉的生物信息学技能。因此，这是一个极好的培训机会，以发展全面的多组分生物科学技能。该项目汇集了脂肪组织(Bath)和肌肉蛋白质合成(Exeter)方面的监督专业知识。研究的主要地点将是巴斯，并根据需要访问埃克塞特。督导团队拥有100%的博士生成功完成记录，巴斯和埃克塞特的研究环境都拥有强大的人体生理学和营养学研究团队。该项目适合对综合人体生理学(广泛)以及饮食(和其他干预策略)感兴趣的人，以减轻老龄化的负面影响。该项目汇集了BBSRC的两个主要优先事项--“食物、营养和健康”和“健康老龄化”。如果饮食中添加特定的营养物质成功地抑制了脂肪炎症并改善了衰老，这将对英国和世界各地数百万老年人产生深远的影响。因此，这个项目处于非常有利的地位，可以在现在和未来产生现实世界的影响。

Demographic shifts in population ageing represent one of the greatest health challenges facing most countries. By 2041, nearly 30% of the UK population will be >65 years old. Thus, finding ways to improve ageing and "healthspan" will be a global priority in the coming decades. Our recent research has shown that adipose tissue from older people is "proinflammatory" even if people remain lean and physically active. Adipose tissue inflammation impacts multiple aspects of health - including the risk of chronic diseases and susceptibility to acute infections. It also impacts the ability to synthesise muscle and thus predisposes people to sarcopaenia, which then causes frailty and reduced functional independence. The aim of this studentship is to explore whether we can use diet to target adipose tissue inflammation and the ability to synthesise muscle in older people. This PhD will combine in vivo and ex vivo approaches with highly sophisticated analytical methods. Laboratory techniques will include; cell culture, advanced 12-colour immuno-phenotyping and cell function measurements with flow cytometry (immunology), multiplex assays, molecular techniques (e.g., RT-PCR, immunoblotting) and stable isotopes (tracers). The studies will be conducted in humans and will include intervention studies (Randomised Controlled Trials); with sophisticated tissue-specific (e.g., adipose and muscle biopsies) and whole-body (e.g., protein turnover) measurements. There will also be the opportunity to develop bioinformatics skills using existing RNAseq datasets in adipose and muscle from older people. Thus, this studentship is an excellent training opportunity to develop well-rounded multicomponent bioscientific skills. The project draws together supervisory expertise in adipose tissue (Bath) and muscle protein synthesis (Exeter). The main location for research will be Bath, with visits to Exeter as required. The supervisory team has a 100% record for the successful completion of PhD students, and the research environment in both Bath and Exeter have strong research teams working in human physiology and nutrition. This studentship would suit someone who is interested in integrative human physiology (broadly) as well as dietary (and other interventional strategies) to mitigate the negative impacts of ageing. This project brings together two major BBSRC priorities - "Food, nutrition and health" and "Healthy Ageing". If dietary enrichment with specific nutrient compounds successfully targets adipose inflammation and improves ageing this would have profound implications given the millions of older people in the UK and around the world. Thus, this project is very well placed to generate real-world impact now and in the future.