权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Microbiota, Probiotic and Dietary Metabolite Control of Enteric Pathogen Virulence

微生物群、益生菌和膳食代谢物对肠道病原体毒力的控制

基本信息

批准号：
10562497
负责人：
Howard C Hang
金额：
$ 77.15万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-11-10 至 2027-10-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10562497
关键词：
ATP phosphohydrolase Acids Affect Animal Model Animals Anti-Infective Agents Anti-Inflammatory Agents Applications Grants Aromatic Amino Acids Attenuated Bacteria Bacterial Infections Bile Acids Biochemical Biology Cell Culture Techniques Cell model Chemicals Chenodeoxycholic Acid Development Diet Gene Expression Genes Genetic Transcription Goals Growth Human Infection Inflammation Intestines Laboratories Mammalian Cell Mammals Methods Modeling Molecular Molecular Target Mucous Membrane Natural Immunity Pathogenesis Pathway interactions Probiotics Property Protein Secretion Proteins Proteomics Regulation Reporting Salmonella typhimurium Site Type III Secretion System Pathway Virulence Virulence Factors Volatile Fatty Acids adaptive immunity combat dietary enteric infection enteric pathogen genetic approach global health in vivo innovation interdisciplinary approach meter microbiota microbiota metabolites mouse model new therapeutic target novel strategies pathogen pathogenic bacteria prevent protein metabolite secondary metabolite therapeutic target transcription factor virulence gene

项目摘要

PROJECT SUMMARY Key metabolites from microbiota, probiotics and diet can inhibit the virulence properties of enteric pathogens, but their mechanisms of action are unclear and limit the development of new anti-infectives. To determine the mechanism(s) of action of prominent microbiota metabolites (short chain fatty acids, aromatic amino acids, bile acids and others), multi-disciplinary approaches are needed to biochemically identify metabolite-protein targets in enteric pathogens and characterize their activity on infections in vivo. My laboratory will therefore employ innovative methods in chemical biology, proteomics and gene editing in bacteria to elucidate how specific metabolites attenuates enteric pathogen infections. A better understanding of how these metabolites affects the virulence properties of bacterial pathogens should reveal potential therapeutic targets and facilitate the development of new anti-infectives to combat bacterial pathogens in animals and humans.

项目摘要来自微生物群、益生菌和饮食的关键代谢物可以抑制肠道病原体的毒力特性，但它们的作用机制尚不清楚，限制了新抗感染药物的开发。确定主要微生物群代谢产物（短链脂肪酸、芳香族氨基酸、胆汁酸）的作用机制酸和其他），需要多学科的方法来生化鉴定代谢物-蛋白质靶标并表征其对体内感染活性。因此，我的实验室将在化学生物学，蛋白质组学和细菌基因编辑的创新方法，以阐明如何具体代谢物减弱肠道病原体感染。更好地了解这些代谢物如何影响细菌病原体的毒力特性应该揭示潜在的治疗靶点，开发新的抗感染药物，以对抗动物和人类的细菌病原体。