Project 5: Molecular biology, chemistry and biochemistry

本计划项目提案的重点是获得最深的理解可能的折叠一个单一的RNA，四膜虫组I核酶。因此，一个中央核心负责创建通用RNA试剂，人员的基本培训以及材料的文档和分发。分子生物学、化学和生物化学（MBCB）项目核心将包括一个分子生物学和化学实验室，配备一名熟练的RNA制备和诱变技术人员。核心是必不可少的生产大量的RNA所需的一些拟议的生物物理实验。RNA的集中生产也将确保所有组件项目的一致性。 核心还将进行常规折叠、稳定性和活性测定，以提供新构建体的基本表征，并证明所有产品均符合所有组件项目要求的质量和纯度水平。

This Program Project proposal is focused on obtaining the deepest understanding possible of the folding of a single RNA, the Tetrahymena group I ribozyme. For this reason, a central Core responsible for the creation of common RNA reagents, the basic training of personnel, and the documentation and distribution of materials. The Molecular Biology, Chemistry and Biochemistry (MBCB) Project Core will consist of a molecular biology and chemistry laboratory with a technical staff skilled in the preparation of RNA and mutagenesis. The core is essential for the production of the large quantities of RNA required for some of the proposed biophysical experiments. The central production of RNA will also insure its consistency for all of the Component Projects. The core will also perform routine folding, stability and activity assays to provide basic characterizations of new constructs and to demonstrate that all products meet a level of quality and purity commensurate with demands of all the Component Projects.