Core--Fluorescence Microscopy Facility

荧光显微镜共享资源的目标是提供活细胞和固定细胞成像所需的设备，软件和专业知识。该设施对用户的影响在很大程度上取决于设备和软件是否容易应用于研究人员提出的实验问题。实现这一目标的办法是，对现有设备进行一致的管理，并确保有足够的人员来制定具体的协议，并调整设备，以解决资源使用者的具体科学问题。 设备/软件：该设施有两个奥林巴斯Fluoview共聚焦显微镜，一个用于低光活细胞成像的Yokagowa旋转圆盘共聚焦显微镜，以及两个配备数码相机的宽视场显微镜。此外，还提供用于制备活样本的设备，包括细胞培养箱和正压罩、离心机和两个立体显微镜。还提供了两台PC工作站，配有用于图像3D渲染、定量和去卷积的软件（例如Improvision的Velocity、Vaytech的PowerMicrotome）。主要设备和软件支持的维护合同确保了用户的持续可用性。 培训/专业知识：该资源提供培训，以支持使用当前的技术和设备，并参加亨斯迈癌症研究所（细胞和分子生物学II）和医学院（光学显微镜和数字成像）的学术课程。主任具有活细胞和固定细胞成像的经验，并继续接受与成像技术相关的培训（例如，活细胞3D成像课程，温哥华，BC，2002）。继续教育是必要的，以保持履行资源使命所需的专业知识水平。 开发/咨询：董事是博士-在荧光细胞成像和图像数据定量分析方面具有丰富经验。图像采集和定量协议的现场开发继续扩大了这一专业范围，旨在促进CCSG成员之间的合作项目。

The objective of the Fluorescence Microscopy Shared Resource is to provide the equipment, software and expertise necessary for the imaging of live and fixed cells. The impact of this facility on its users is largely dependent on how easily the equipment and software can be applied to the experimental questions posed by the researcher. This objective is fulfilled by providing consistent management of current equipment and ensuring that sufficient personnel are available to develop specific protocols and adapt equipment to address the specific scientific questions of resource users. Equipment/Software: The facility has two Olympus Fluoview confocal microscopes, a Yokagowa spinning disk confocal for low-light live cell imaging, and two widefield microscopes equipped with digital cameras. In addition, equipment for preparing live samples is available, including a cell culture incubator and positive pressure hood, centrifuge, and two stereomicroscopes. Two PC workstations with software for 3D rendering, quantitation and deconvolution of images (e.g. Velocity from Improvision, PowerMicrotome from Vaytech) are also provided. Maintenance contracts for major equipment and software support ensures consistent availability for users. Training/Expertise: The resource provides training to support use of current techniques and equipment and takes part in academic courses at Huntsman Cancer Institute (Cell and Molecular Biology II) and the School of Medicine (Light Microscopy and Digital Imaging). The director has experience imaging live and fixed cells and continues to receive training relevant to imaging techniques (e.g., a course in 3D imaging of live cells,Vancouver, BC, 2002). Continued education is required to maintain the level of expertise necessary to fulfill the resource mission. Development/Consultation: The director is a Ph.D.-level biologist with extensive experience in fluorescent cell imaging and quantitative analysis of image data. On-site development of image acquisition and quantitation protocols continues to expand this range of expertise, and is designed to promote collaborative projects among CCSG members.