Reuteri LuxS in Probiosis for Early Childhood Caries

描述：本研究旨在探讨变形链球菌益生菌挑战过程中群体感应在种间交流中的作用。关键目标是确定LuxS和自诱导剂2型（AI-2）介导的信号在益生菌罗伊氏乳杆菌（reuteri）和口腔病原体变形链球菌（mutans）相互作用中的作用。实验室和患者衍生的变形菌株将在罗伊氏菌存在下测试对变形菌生物膜形成和变形菌生物膜相关基因表达的影响。研究将强调益生菌罗伊氏菌作为早期儿童龋齿相关口腔生物膜的可能治疗策略的作用。通过2年的研究，我们的目标是增加我们对luxs依赖途径和益生菌lactobacillus - derus - AI-2在控制蛀牙突变体生物膜形成和定植中的理解。探讨罗伊氏乳杆菌（reuteri） luxs依赖性信号及其对变形链球菌（mutans） luxs依赖性生物膜形成的影响。2. 探讨罗伊氏乳杆菌（reuteri） LuxS在幼儿龋齿相关变形链球菌（mutans）生物膜相关基因表达和生物膜形成调控中的作用。

DESCRIPTION: This study is designed to explore the role of interspecies communication by quorum sensing during probiotic challenge of Streptococcus mutans. The key goal is to determine the role of LuxS and autoinducer type-2 (AI-2)-mediating signaling during interactions of the probiotic bacterium, Lactobacillus reuteri (Reuteri), and the oral pathogen, Streptococcus mutans (Mutans). Laboratory and patient-derived Mutans strains will be tested in the presence of Reuteri for effects on Mutans biofilm formation and Mutans biofilm-associated gene expression. The investigations will emphasize the role of probiotic Reuteri as a possible therapeutic strategy for oral biofilms associated with early childhood caries. By the end of the 2-year study, we Aim to increase our understanding of LuxS-dependent pathways and probiotic Lactobacillus-derwed AI-2 in the control of biofilm formation and colonization by cariogenic Mutans. 1. Explore Lactobacillus reuteri (Reuteri) LuxS-dependent signaling and its effects on Streptococcus mutans (Mutans) LuxS-dependent biofilm formation. 2. Investigate the role of Lactobacillus reuteri (Reuteri) LuxS in regulation of biofilm-related gene expression and biofilm formation by early childhood caries-associated Streptococcus mutans (Mutans).