Structural Studies of Virus and Receptor Interactions

描述(由申请人提供)： 这项建议的总体目标是了解病毒和受体的相互作用。将确定小的无包膜病毒与细胞成分相互作用的三维结构，这些细胞成分在细胞识别、进入或病毒脱壳期间调节病毒的生命周期。对特定细胞受体的识别是确定宿主范围和组织趋向性的重要组成部分。病毒已经进化到使用大量的细胞表面分子作为受体，一些病毒使用常见的化学基团，而另一些病毒识别非常特定的分子。因此，受体的分布将决定病毒的趋向性和感染的症状。在这里提出的研究中，将使用两种不同的病毒系统：阳性单链单链RNA小核糖核酸病毒，包括ECHO病毒7和柯萨奇B3病毒；以及含有单链DNA的细小病毒，包括猫科成员泛白细胞减少症病毒和犬细小病毒。这两个病毒家族都是简单的、无包膜的二十面体病毒，其外径约为300埃。

DESCRIPTION (provided by applicant): The overall goal of this proposal is directed at understanding virus and receptor interactions. Three- dimensional structures will be determined of small non-enveloped viruses interacting with cellular components that modulate the viral life cycle during cell recognition, entry or viral uncoating. Recognition of specific cellular receptors is a significant part of the determination of host range and tissue tropism. Viruses have evolved to use a large variety of cell-surface molecules as receptors with some viruses using commonly found chemical groups whereas other viruses recognize very specific molecules. Hence, the distribution of the receptor will determine the tropism of the virus and the symptoms of the infection. For the research proposed here, two different viral systems will be used: positive stranded ssRNA picornaviruses, including echovirus 7 and coxsackieB3; also the ssDNA-containing parvoviruses, including family members feline panleukopenia virus and canine parvovirus. Both families of viruses are simple, non-enveloped, icosahedral viruses with an external diameter of approximately 300 Angstroms.