Pyk2 Signaling In Intestinal Epithelial Cells

续页首席研究员/项目主任（上、一、中）：吴世芳除了Enrique Rozengurt博士在信号转导和生长调节领域的直接指导外，吴博士还将受益于他的科学咨询委员会的建议和指导，该委员会由我、消化内科副教授Martin Martin博士和新生儿科主任Sherin Devaskar教授组成。研究副主席。我们都非常致力于指导研究员和年轻教师。自1991年以来，琳达·麦凯布博士和我每年都开设一门名为“如何在学术上取得成功”的课程，我们还出版了一本同名的书。从1996年开始，我们还发起了一个领导力研讨会。我们绝对致力于指导，吴博士的委员会是以指导为核心价值的个人的典范。自1997年被招募到加州大学洛杉矶分校以来，Rozengurt博士已经获得了三次NIDDK R01资助和NCI P50 SPORE机构项目资助，并在医学系消化疾病部建立了一个杰出的、富有成效的实验室小组。除了吴博士，这个实验小组包括四名研究生，其中三名是博士后。研究员8名，博士后8名，兼职本科生3名，副研究员1名，技术人员1名。该实验室占地5000平方英尺，位于沃伦大厅的一楼，这里有营养系和医学系的几个部门的研究空间。该实验室设备齐全，可进行信号转导研究，有两个细胞培养罩，多个孵化器，冰箱和冰柜，包括液氮细胞储存，以及蛋白质和核苷酸电泳和凝胶转移设备。有一个用于测量细胞内钙通量的分光光度计，一个带x射线显影机的暗室，一个用于放射性工作和储存的单独房间，包括一个液体闪烁计数器。该实验室在大楼内共享高分辨率视频显微镜系统，并在加州大学洛杉矶分校校园的其他地方共享分子生物学研究所的核心资源，包括共聚焦显微镜设备和CURE/消化疾病研究中心。作为他职业发展和培训的重要组成部分，吴博士之前参加了我们为期两天的“如何在学术上取得成功”研讨会，并将参加未来为期一天的“学术领导力”研讨会，以及由Devaskar博士开发的一系列每周中午会议。这些会议在以下主题之间轮换：建立成功的研究生涯的教学培训；正在进行的研讨会，由研究员和初级教员作报告；由资深教员主持的院系研究研讨会；还有一个儿科研究期刊俱乐部。他还将参加每周一次的消化疾病杂志俱乐部和两周一次的信号转导小组研讨会。他将参加在其提案的候选人背景部分中概述的研究生水平课程，这将丰富他对分子生物学和遗传学的理解。他将继续在实验室会议、部门会议和国家会议上展示他的研究，如美国胃肠病学协会（消化疾病周）和北美儿科胃肠病学、肝病学和营养学会（NASPGHAN）。来自Rozengurt博士实验室和儿科的资源组合，将为吴医生提供智力和体力资源，支持和刺激培训计划，就像我们以前为许多在学术医学上取得成功的学员提供的那样。根据我以前在加州大学洛杉矶分校和其他机构培训的学员的经验，像吴博士这样的背景、技能和动机的学员已经成为真正优秀的内科科学家。KO8资助将有助于他开启学术生涯。作为儿科系主任，我将继续保护他在K08的五年任期内80%的研究时间，如果他选择留在加州大学洛杉矶分校的话。我相信吴医生将在他的研究方法上富有成效和创新，并将继续发展成为儿科胃肠病学领域杰出的独立医生和科学家。PHS 398 （Rev. 4/98）第21页使用%-英寸页边距。在整个应用程序的底部连续编号页面。家用后缀如3a， 3b。======================================== 节结束 ===========================================

CONTINUATIONPAGE Principal Investigator/PrograrnDirector (Last, first,middle): Wu, Steven Shi-fong 5. Environmental and Institutional Commitmentto the Candidate a. Description of the Institutional Environment In addition to direct mentoring from Dr. Enrique Rozengurt in the fields of signal transduction and growth regulation, Dr. Wu will benefit from the advice and guidance of his ScientificAdvisory Committee, consisting of myself, Dr. Martin Martin, Associate Professor in the Division of Gastroenterology, and Dr. Sherin Devaskar, Professor, Chiefof the Division ofNeonatology, and Vice Chair for Research. We are all very committed to mentoring fellows and young faculty. Dr. Linda McCabe and I have been offering an annual course, How to Succeed in Academics, since 1991,and we have published a book of the sametitle. We also initiated a Leadership Workshop beginning in 1996. We are absolutely committed to mentoring and Dr. Wu's committee exemplifies individuals for whom this is a central value. Since being recruited to the UCLA faculty in 1997, Dr. Rozengurt has been the recipient of three NIDDK R01 grants and a section of an NCI P50 SPORE institutional project grant, and has established an outstanding and productive laboratory group within the Division of Digestive Diseases, Department of Medicine. In addition to Dr. Wu, this laboratory group includes four graduate students of whom three are post-M.D. fellows, eight postdoctoral Ph.D. fellows, three part-time undergraduate students, one research associate, and one technician. The laboratory covers 5,000 square feet on the first floor of Warren Hall, which houses research space for the Department of Nutrition and several divisions of the Department of Medicine. The laboratory is fully equipped for signal transduction research with two cell culture hoods, multiple incubators, refrigerators, and freezers including liquid nitrogen cell storage, as well as equipment for protein and nucleotide electrophoresis and gel transfer. There is a spectrophotometer for measuring intracellular calcium flux, a darkroom with an X-ray film developing machine, and a separate room for radioactive work and storage, and including a liquid scintillation counter. The laboratory shares access within the building to a high resolution videomicroscopy system and elsewhere on the UCLA campus to the core resources of the Molecular Biology Institute, including confocal microscopy equipment, and of the CURE/Digestive Diseases Research Center. As an important part of his career development and training, Dr. Wu has previously attended our two- day workshop on How to Succeed in Academics, and will attend a future one-day workshop on Academic Leadership, in addition to a series of weekly noon conferences developed by Dr. Devaskar. These conferences rotate between the following topics: Didactic training in establishing a successful research career; a work-in-progress seminar with presentations by fellows andjunior faculty; a departmental research seminar presented by senior faculty; and a pediatric researchjournal club. He will also attend a weekly Division of Digestive Diseasesjournal club and a biweekly Signal Transduction Group seminar. He will enroll in the graduate level courses outlined in the Candidate Background section of his proposal, which will enrich his understanding of molecular biology and genetics. He will continue to present his research in lab meetings, departmental meetings, and at national meetings such as those of the American Gastroenterological Association (Digestive Disease Week) and ofthe North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition (NASPGHAN). This combination of resources, from both Dr. Rozengurt's laboratory and the Department of Pediatrics, will provide the intellectual and physical resources for a supportive and stimulatingtraining program for Dr. Wu, as have been provided for many of our previous trainees who are successful in academic medicine. In my experience with previous trainees at UCLA and other institutions,trainees with Dr. Wu's background, skills, and motivation have become truly superb physician-scientists. A KO8 grant will be instrumental in launching his academic career. As Chair of the Department of Pediatrics, I will continue to protect his research time at 80% throughout his five-year tenure on the K08, and beyond, if he elects to remain at UCLA. I am confident that Dr. Wu will be productive and innovative in his approach to research, and will continue to develop into an outstanding independent physician-scientist in the field ofpediatric gastroenterology. PHS 398 (Rev. 4/98) Page 21 Use %-inch MARGINS. Number pages consecutively at the bottomthroughout the application. Domusesuffixes such as 3a, 3b. ========================================Section End===========================================