THE ROLE OF LFA-1 IN T CELL ACTIVATION

说明(改编自应用程序) 克拉拉·亚伯拉罕，医学博士，获得计算机科学学士学位 1985年在伊利诺伊大学获得工程学院荣誉， Urbana，1989年在普利兹克芝加哥大学获得医学学位 医学院。在拉什的内科实习医生之后 长老会-圣彼得堡卢克医疗中心，她完成了三年的基础 芝加哥大学胃肠病学科学奖学金。在.期间 在此期间，她进入托马斯·A·布拉西图斯博士的实验室，研究 蛋白激酶C和1，25(OH)2D3在细胞生长、分化和分化中的作用 Caco-2人结肠癌细胞凋亡的研究接下来的三年 作为奖学金，她进入了吉姆·米勒博士的实验室，以便进行 基础科学免疫学方面的额外培训。她一直在调查 近3年来LFA-I在T淋巴细胞活化中的作用 在目前的提案中，亚伯拉罕博士计划继续提出关于 LFA-1在体内外对T细胞功能的影响具体地说，她打算 为了进一步理解和区分特定职能的贡献 通过体外诱变激活T细胞内的LFA-I。到时候她会的 利用在体外获得的信息来鉴定特定的、戏剧性的T细胞 在体内炎症模型中要分析的表型，特别是， 炎症性肠病小鼠模型。这种方法的目标是 允许分离LFA-L对T细胞转运的贡献与T细胞 细胞激活，并评估这些功能在体内的相对重要性。 由于亚伯拉罕博士没有接受过IBD和肠道动物模型的正式培训 免疫学，拟议的研究职业发展计划要求支持 五年，以提供正式的、全面的培训计划 这将使她能够建立一个独立的实验室来追求 在这些体内炎症模型中的研究。

DESCRIPTION (adapted from the application) Clara Abraham, MD, received a baccalaureate degree in computer science from the College of Engineering, high honors, in 1985 at the University of Illinois, Urbana, and a medical degree in 1989 at the University of Chicago, Pritzker School of Medicine. Following internal medicine residency at Rush Presbyterian-St. Luke's Medical Center, she completed a three year basic science fellowship in gastroenterology at The University of Chicago. During this period, she entered the laboratory of Dr. Thomas A. Brasitus, and studied the role of protein kinase C and 1,25(OH)2D3 in the growth, differentiation and apoptosis of Caco-2 human colon cancer cells. Following the three year fellowship, she entered the laboratory of Dr. Jim Miller in order to pursue additional training in basic science immunology. She has been investigating the role of LFA- I in the activation of T lymphocytes for the past 3 years. In the present proposal, Dr. Abraham plans to pursue questions regarding the in vitro and in vivo role of LFA-1 in T cell function. Specifically, she intends to further understand and segregate the contributions of specific functions of LFA-I within T cell activation through in vitro mutagenesis. She will then utilize the information acquired in vitro to identify specific, dramatic T cell phenotypes to be analyzed in in vivo models of inflammation, in particular, murine models of inflammatory bowel disease. The goal of such an approach is to allow for segregation of LFA-l's contribution to T cell trafficking versus T cell activation, and assess the relative importance of these functions in vivo. As Dr. Abraham has no formal training in animal models of IBD and intestinal immunology, the proposed research career development plan requests support for five years in order to provide a formalized, comprehensive training program for her that will allow her to establish an independent laboratory to pursue research in these in vivo models of inflammation.