CORE--MOLECULAR BIOLOGY

路易斯安那州立大学COBRE核心实验室（LSU-MBCL）将在生物技术和分子医学部（BIOMMED）和路易斯安那州立大学兽医学院病理生物科学系的现有集中设施内运行。LSU-MBCL将集中支持分子生物学/重组DNA服务，生物信息学和小鼠免疫参数的分子评估。分子生物学服务和试剂将包括DNA测序、重组质粒和cDNA文库构建等标准服务。先进的服务将包括使用先进的生物信息学能力与路易斯安那州立大学彭宁顿生物医学研究中心（PBRC-BU）的生物信息学单位一起提供的微阵列实验的处理和分析。此外，LSU-MBCL将提供小鼠细胞因子的实时PCR分析，荧光激活分选（FACS）分析和共聚焦显微镜合作 与兽医学院集中设施致力于流式细胞仪和光学显微镜。LSU-MBCL的具体目标是：（1）为COBRE研究人员提供先进的分子生物学知识，试剂和服务，包括生物信息学。(2)促进使用先进的分子免疫测定法来评估小鼠的免疫反应，以及使用路易斯安那州立大学兽医学院的其他研究资源。

The Louisiana State University COBRE Core laboratory (LSU-MBCL) will be operationally housed within the existing centralized facilities of the Division of Biotechnology and Molecular Medicine (BIOMMED) and the Department of Pathobiological Sciences at the LSU School of Veterinary Medicine. The LSU-MBCL will concentrate its support functions on molecular biology/recombinant DNA services, bioinformatics and molecular evaluation of immunological parameters of mice. Molecular biological services and reagents will include standard services such as DNA sequencing, recombinant plasmid and cDNA library construction. Advanced services will include processing and analysis of microarray experiments using advanced bioinformatics capabilities offered in conjunction with the Bioinformatics Unit of the LSU Pennington Biomedical Research Center (PBRC-BU). In addition, LSU-MBCL will offer real-time PCR analysis of mouse cytokines, Fluorescent Activated Sorting (FACS) analysis, and confocal microscopy in collaboration with the School of Veterinary Medicine Centralized Facilities dedicated to FACS and Light Microscopy. The specific aims of the LSU-MBCL are: (1) To provide advanced molecular biological know-how, reagents and services including bioinformatics to COBRE investigators. (2) To facilitate the use of advanced molecular immunoassays for the evaluation of immune responses of mice, and the use of other research resources of the LSU School of Veterinary Medicine.