LSU VETERINARY COBRE: MOLECULAR BIOLOGY AND IMMUNOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 路易斯安那州立大学科布雷核心实验室(LSU-MBIC)位于路易斯安那州立大学兽医学院生物技术和分子医学部(BIOMMED)现有的集中设施内。LSU-MBIC的支持功能主要集中在分子生物学/重组DNA服务、生物信息学和小鼠免疫学参数的分子评估等方面。分子生物学服务和试剂包括标准服务，如DNA测序、实时聚合酶链式反应、重组质粒构建和纯化以及cDNA文库构建。先进的服务包括使用与路易斯安那州立大学彭宁顿生物医学研究中心(PBRC-BU)的生物信息学单位联合提供的先进生物信息学能力来处理和分析微阵列实验。此外，LSU-MBIC还与兽医学院合作，提供了小鼠细胞因子的实时PCR分析、荧光激活分类(FACS)分析和共聚焦显微镜，并提供了专门用于FACS和光学显微镜的中央设施。LSU-MBIC的具体目标是：(1)向Cobre研究人员提供先进的分子生物学知识、试剂和服务，包括生物信息学。(2)促进使用先进的分子免疫分析方法评估小鼠的免疫反应，以及使用路易斯安那州立大学兽医学院的其他研究资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Louisiana State University COBRE Core laboratory (LSU-MBIC) is operationally housed within the existing centralized facilities of the Division of Biotechnology and Molecular Medicine (BIOMMED) at the LSU School of Veterinary Medicine. The LSU-MBIC has concentrated its support functions on molecular biology/recombinant DNA services, bioinformatics and molecular evaluation of immunological parameters in mice. Molecular biological services and reagents have included standard services such as DNA sequencing, real-time PCR runs, recombinant plasmid construction and purification, and cDNA library construction. Advanced services have included processing and analysis of microarray experiments using advanced bioinformatics capabilities offered in conjunction with the Bioinformatics Unit of the LSU Pennington Biomedical Research Center (PBRC-BU). In addition, LSU-MBIC has offered real-time PCR analysis of mouse cytokines, Fluorescent Activated Sorting (FACS) analysis, and confocal microscopy in collaboration with the School of Veterinary Medicine Centralized Facilities dedicated to FACS and Light Microscopy. The specific aims of the LSU-MBIC are: (1) To provide advanced molecular biological know-how, reagents and services including bioinformatics to COBRE investigators. (2) To facilitate the use of advanced molecular immunoassays for the evaluation of immune responses of mice, and the use of other research resources of the LSU School of Veterinary Medicine.