Purchase of Confocal Laser Scanning Microscope

描述(由申请人提供)：为拉霍亚分子医学研究所的核心显微镜成像设施申请购买激光扫描共聚焦显微镜。虽然有几台现代荧光显微镜可供工作人员普遍使用，但我们在这个地点没有共焦显微镜。鉴于La Jolla地区对使用其他共焦显微镜的收费要求很高，以及运输许多样本(即活体成像、活细胞、新鲜组织标本)的时间敏感性和难度，增加一台校园共焦显微镜将极大地增强NIH资助的一系列不同的研究项目。例如，由于检疫和手术需要的困难，在校园外对植入动物模型的活体房间进行成像是不可行的。如本提案所述，我们成立了一个委员会(Brian Eliceiri、Carole Banka、Eugene Levin和Ingrid Schraufstatter)，以确定主要用户的核心群体，确定研究所的具体需求，并选择适当的工具来实现我们研究小组的目标。这种共聚焦显微镜的操作将集成到一个位于中心的开放式核心成像设备中，该设备已建立用于活体成像以及时间进程细胞迁移和普通显微镜。 在广泛咨询和分析了各种竞争对手的共焦显微镜系统后，我们确定奥林巴斯FluoView 500是核心环境的理想系统，最大限度地提高了易用性，具有满足当前和未来要求的强大规格。七个主要用户中有六个通过NIH有活跃的R01资助的研究计划，第七个研究人员得到其他来源的支持，RO1已提交并等待审查。所申请的共焦显微镜的成本为274,579美元，并将与之相匹配的是，机构承诺每年10,000美元的服务合同，未来更改/升级的全部成本，以及为管理显微镜的技术人员提供80%的工资支持。工资的余额将来自根据使用情况从个人补助金中重新获得的成本。与我们现有的成像基础设施相结合，拟议的共焦显微镜将是一个重要的补充，以最大限度地发挥我们现有研究计划的影响。

DESCRIPTION (provided by applicant): The purchase of a laser scanning confocal microscope is requested for the core microscope imaging facility at the La Jolla Institute for Molecular Medicine. While several modern fluorescent microscopes are available for general use by the staff, we do not have a confocal microscope at this site. Based upon the high demand placed on the fee-for-service access to other confocal microscopes in the La Jolla area and the time-sensitive nature and difficulty in transporting many samples (i.e. intravital imaging, live cells, fresh tissue specimens), the addition of an on-campus confocal microscope would greatly enhance a diverse array of ongoing NIH-funded research programs. For example, imaging of intravital chambers implanted in animal models is not feasible off-campus due to the difficulties of quarantine and surgical needs. As detailed in this proposal, we have formed a committee (Brian Eliceiri, Carole Banka, Eugene Levin, and Ingrid Schraufstatter) to identify a core group of Primary Users, determined the specific needs of the institute, and chosen appropriate instrumentation to accomplish the goals of our research group. The operation of this confocal microscope will be integrated into a centrally located, open access core imaging facility that has been established for in vivo imaging as well as time course cell migration and general microscopy. Following extensive consultation and analysis of various competing confocal microscope systems, we have identified the Olympus Fluoview 500 as the ideal system for a core environment, maximizing ease of use with capable specifications for current and future requirements. Six of the seven Primary Users have active R0l-funded research programs through the NIH, with the seventh investigator supported by other sources and an RO1 submitted and pending review. The cost of the requested confocal microscope is $274,579 and will be matched by an institutional commitment for an annual $10,000 serve contract, the full cost of future changes/upgrades, and 80% salary support for a technician to manage the microscope. The balance of the salary will come from a cost-recapture from individual grants, based on usage. In combination with our existing imaging infrastructure, the proposed confocal microscope will be an important addition to maximize the impact of our existing research programs.